Base and prime editors (BEs and PEs) may provide more precise genetic engineering than
nuclease-based approaches because they bypass the dependence on DNA double-strand …

Cytosine base editors (CBEs) enable targeted C• G-to-T• A conversions in genomic DNA.
Recent studies report that BE3, the original CBE, induces a low frequency of genome-wide …

Gene therapy is limited by inefficient delivery of large clustered regularly interspaced short
palindromic repeat (CRISPR) effectors, such as Cas9 and Cas12a nucleases. Cas12f …

For precise genome editing via CRISPR/homology-directed repair (HDR), effective and safe
editing of long-term engrafting hematopoietic stem cells (LT-HSCs) is required. The impact …

The CRISPR/Cas system has emerged as a powerful and versatile genome engineering
tool, revolutionizing biological and biomedical sciences, where an improvement of efficiency …

Mutational outcomes following CRISPR-Cas9-nuclease cutting in mammalian cells have
recently been shown to be predictable and, in certain cases, skewed toward single …

CRISPR/Cas9-mediated mutagenesis typically results in short insertion/deletion mutations,
which are often too small to disrupt the function of cis-acting regulatory elements. Here, we …

Retinitis pigmentosa (RP) consists of a group of inherited, retinal degenerative disorders
and is characterized by progressive loss of rod photoreceptors and eventual degeneration of …

Adenine base editors (ABEs) have been subjected to multiple rounds of mutagenesis with
the goal of optimizing their function as efficient and precise genome editing agents. Despite …

Strategies to modulate cellular DNA repair pathways hold immense potential to enhance the
efficiency of CRISPR-Cas9 genome editing platform. In the absence of a repair template …