Saccharomyces cerevisiae, Baker's yeast, is the industrial workhorse for producing ethanol
and the subject of substantial metabolic engineering research in both industry and …

The goal of this paper is to review and critically assess different methods to monitor key
process variables for ethanol production from lignocellulosic biomass. Because cellulose …

Background The four-carbon dicarboxylic acids of the tricarboxylic acid cycle (malate,
fumarate and succinate) remain promising bio-based alternatives to various precursor …

This work proposes a hybrid observer based on an asymptotic observer combined with an
extended Kalman filter applied to high-density cultures of S. cerivisiae. The observer …

Accurate control of the specific growth rate (µ) of microorganisms is dependent on the ability
to quantify the evolution of biomass reliably in real time. Biomass concentration can be …

High-cell density cultures (HCDC), prone to metabolic overflow, are typically operated in fed-
batch mode to maximize productivity. We developed a simulation-based procedure for …

Background Microbial metabolism is highly dependent on the environmental conditions.
Especially, the substrate concentration, as well as oxygen availability, determine the …

Mini-bioreactor systems enabling automatized operation of numerous parallel cultivations
are a promising alternative to accelerate and optimize bioprocess development allowing for …

Background In industrial microbial biotechnology, fed-batch processes are frequently used
to avoid undesirable biological phenomena, such as substrate inhibition or overflow …

Yeast is a dominant host for recombinant production of heterologous proteins, high-value
biochemical compounds, and microbial fermentation. During bioprocess operations, pH …