Nanomedicine is an emerging field that exploits nanotechnology for the development of
novel therapeutic and diagnostic modalities. Researches are been focussed in nanoimaging …

Understanding the interactions between nanomaterials and biological systems plays an
essential role in enhancing the efficacy of nanomedicines and deepening the understanding …

The endomembrane system consists of the secretory and endocytic pathways, which
communicate by transport to and from the trans-Golgi network (TGN). In mammalian cells …

Golgi cisternal maturation has been visualized by fluorescence imaging of individual
cisternae in the yeast Saccharomyces cerevisiae, but those experiments did not track …

Saccharomyces cerevisiae is amenable to studying membrane traffic by live-cell
fluorescence microscopy. We used this system to explore two aspects of cargo protein traffic …

The pathways of membrane traffic within the Golgi apparatus are not fully known. This
question was addressed using the yeast Saccharomyces cerevisiae, in which the maturation …

The progression in contemporary scientific field is facilitated by a multitude of sophisticated
and cutting‐edge methodologies that are employed for various research purposes. Among …

Significance Cellular metabolic dynamics can occur within milliseconds, yet there are no
optimal tools to spatially and temporally capture these events. Autofluorescence imaging …

Deconvolution microscopy is a very useful, software-based technique allowing to deblur
microscopy images and increase both lateral and axial resolutions. It can be used along with …

LONG ABSTRACT The goal of this protocol is to characterize how membrane compartments
form and transform in live cells of budding yeast. Many intracellular compartments in yeast …