Xanthene, cyanine, oxazine and BODIPY: the four pillars of the fluorophore empire for super-resolution bioimaging

S Samanta, K Lai, F Wu, Y Liu, S Cai, X Yang… - Chemical Society …, 2023 - pubs.rsc.org
In the realm of biological research, the invention of super-resolution microscopy (SRM) has
enabled the visualization of ultrafine sub-cellular structures and their functions in live cells at …

Organelle-targeting ratiometric fluorescent probes: design principles, detection mechanisms, bio-applications, and challenges

MK Goshisht, N Tripathi, GK Patra, M Chaskar - Chemical Science, 2023 - pubs.rsc.org
Biological species, including reactive oxygen species (ROS), reactive sulfur species (RSS),
reactive nitrogen species (RNS), F−, Pd2+, Cu2+, Hg2+, and others, are crucial for the …

Parental genome unification is highly error-prone in mammalian embryos

T Cavazza, Y Takeda, AZ Politi, M Aushev, P Aldag… - Cell, 2021 - cell.com
Most human embryos are aneuploid. Aneuploidy frequently arises during the early mitotic
divisions of the embryo, but its origin remains elusive. Human zygotes that cluster their …

Genetically encoded multimeric tags for subcellular protein localization in cryo-EM

HKH Fung, Y Hayashi, VT Salo, A Babenko, I Zagoriy… - Nature …, 2023 - nature.com
Cryo-electron tomography (cryo-ET) allows for label-free high-resolution imaging of
macromolecular assemblies in their native cellular context. However, the localization of …

A general highly efficient synthesis of biocompatible rhodamine dyes and probes for live-cell multicolor nanoscopy

J Bucevičius, R Gerasimaitė, KA Kiszka… - Nature …, 2023 - nature.com
The development of live-cell fluorescence nanoscopy is powered by the availability of
suitable fluorescent probes. Rhodamines are among the best fluorophores for labeling …

Stimulated emission depletion microscopy

G Lukinavičius, J Alvelid, R Gerasimaitė… - Nature Reviews …, 2024 - nature.com
In this Primer, we focus on the most recent advancements in stimulated emission depletion
(STED) microscopy, encompassing optics, computational microscopy and probes design …

Advancing cell biology with nanoscale fluorescence imaging: essential practical considerations

E DʼEste, G Lukinavičius, R Lincoln, F Opazo… - Trends in Cell …, 2024 - cell.com
Recently, biologists have gained access to several far-field fluorescence nanoscopy (FN)
technologies that allow the observation of cellular components with~ 20 nm resolution. FN is …

Asynchronous nuclear cycles in multinucleated Plasmodium falciparum facilitate rapid proliferation

S Klaus, P Binder, J Kim, M Machado, C Funaya… - Science …, 2022 - science.org
Malaria-causing parasites proliferate within erythrocytes through schizogony, forming
multinucleated stages before cellularization. Nuclear multiplication does not follow a strict …

Recent advances in organelle-targeted fluorescent probes

NE Choi, JY Lee, EC Park, JH Lee, J Lee - Molecules, 2021 - mdpi.com
Recent advances in fluorescence imaging techniques and super-resolution microscopy
have extended the applications of fluorescent probes in studying various cellular processes …

[PDF][PDF] An extended DNA-free intranuclear compartment organizes centrosome microtubules in malaria parasites

CS Simon, C Funaya, J Bauer, Y Voβ… - Life science …, 2021 - life-science-alliance.org
Plasmodium falciparum encounters significant population bottlenecks when being
transmitted between humans and mosquitoes. To overcome those, it undergoes several …