Functional evidence supports adaptive plant chemical defense along a geographical cline

AA Agrawal, L Espinosa del Alba… - Proceedings of the …, 2022 - National Acad Sciences
Environmental clines in organismal defensive traits are usually attributed to stronger
selection by enemies at lower latitudes or near the host's range center. Nonetheless, little …

Cardenolides, toxicity, and the costs of sequestration in the coevolutionary interaction between monarchs and milkweeds

AA Agrawal, K Böröczky, M Haribal… - Proceedings of the …, 2021 - National Acad Sciences
For highly specialized insect herbivores, plant chemical defenses are often co-opted as cues
for oviposition and sequestration. In such interactions, can plants evolve novel defenses …

Plant defense by latex: Ecological genetics of inducibility in the milkweeds and a general review of mechanisms, evolution, and implications for agriculture

AA Agrawal, AP Hastings - Journal of Chemical Ecology, 2019 - Springer
Latex occurs in 10% of plant families, has evolved independently many times, and is the
most effective defense of milkweeds against its chewing herbivores. Here we report on new …

Independent evolution of ancestral and novel defenses in a genus of toxic plants (Erysimum, Brassicaceae)

T Züst, SR Strickler, AF Powell, ME Mabry, H An… - Elife, 2020 - elifesciences.org
Phytochemical diversity is thought to result from coevolutionary cycles as specialization in
herbivores imposes diversifying selection on plant chemical defenses. Plants in the …

Quantification of plant cardenolides by HPLC, measurement of Na+/K+-ATPase inhibition activity, and characterization of target enzymes

G Petschenka, T Züst, AP Hastings, AA Agrawal… - Methods in …, 2023 - Elsevier
The biosynthesis of cardiac glycosides, broadly classified as cardenolides and
bufadienolides, has evolved repeatedly among flowering plants. Individual species can …

A nutrition–defence trade-off drives diet choice in a toxic plant generalist

NJ Carlson, AA Agrawal - Proceedings of the Royal …, 2023 - royalsocietypublishing.org
Plant toxicity shapes the dietary choices of herbivores. Especially when herbivores
sequester plant toxins, they may experience a trade-off between gaining protection from …

The price of defence: toxins, visual signals and oxidative state in an aposematic butterfly

JD Blount, HM Rowland, C Mitchell… - … of the Royal …, 2023 - royalsocietypublishing.org
In a variety of aposematic species, the conspicuousness of an individual's warning signal
and the quantity of its chemical defence are positively correlated. This apparent honest …

Plant water‐use strategies as mediators of herbivore drought response: Ecophysiology, host plant quality and functional traits

AN Carvajal Acosta, AA Agrawal… - Journal of …, 2023 - Wiley Online Library
The response of herbivorous insects to plant drought stress can range from positive to
negative, and it has been challenging to understand the causes of this variation. We tested …

Adaptive substitutions underlying cardiac glycoside insensitivity in insects exhibit epistasis in vivo

AM Taverner, L Yang, ZJ Barile, B Lin, J Peng… - Elife, 2019 - elifesciences.org
Predicting how species will respond to selection pressures requires understanding the
factors that constrain their evolution. We use genome engineering of Drosophila to …

Cardenolide intake, sequestration, and excretion by the monarch butterfly along gradients of plant toxicity and larval ontogeny

PL Jones, G Petschenka, L Flacht… - Journal of Chemical …, 2019 - Springer
Monarch butterflies, Danaus plexippus, migrate long distances over which they encounter
host plants that vary broadly in toxic cardenolides. Remarkably little is understood about the …