Single-molecule super-resolution fluorescence microscopy and single-particle tracking are
two imaging modalities that illuminate the properties of cells and materials on spatial scales …

Optical microscopy has for centuries been a key tool to study living cells with minimum
invasiveness. The advent of single molecule techniques over the past two decades has …

To extract from an image of a single nanoscale object maximum physical information about
its position, we propose and demonstrate a framework for pupil-plane modulation for 3D …

Newly developed scientific complementary metal-oxide semiconductor (sCMOS) cameras
have the potential to dramatically accelerate data acquisition, enlarge the field of view and …

Super-resolution localization microscopy methods provide powerful new capabilities for
probing biology at the nanometer scale via fluorescence. These methods rely on two key …

Single-molecule biophysics has transformed our understanding of biology, but also of the
physics of life. More exotic than simple soft matter, biomatter lives far from thermal …

Estimation of a parameter of interest from image data represents a task that is commonly
carried out in single molecule microscopy data analysis. The determination of the positional …

Highlights•Molecular specificity and live-cell compatibility are key fluorescence
strengths.•Time-sequential imaging and precise localization of sparse single-molecule …

A study of the uncertainty of localizing single‐molecule emitters for super‐resolution light
microscopy is presented. Maximum likelihood estimation (MLE) is found to be superior to …

A novel interferometric method–SLIVER (Super Localization by Image inVERsion
interferometry)–is proposed for estimating the separation of two incoherent point sources …