Chemically modified biomacromolecules─ ie, proteins, nucleic acids, glycans, and lipids─
have become crucial tools in chemical biology. They are extensively used not only to …

The formation of new blood vessels, or angiogenesis, is a complex process that plays
important roles in growth and development, tissue and organ regeneration, as well as …

Next-generation sequencing technology has rapidly accelerated the discovery of genetic
variants of interest in individuals with rare diseases. However, showing that these variants …

Zebrafish is a powerful model for forward genetics. Reverse genetic approaches are limited
by the time required to generate stable mutant lines. We describe a system for gene …

Extracellular vesicles (EVs) are released by most cell types but providing evidence for their
physiological relevance remains challenging due to a lack of appropriate model organisms …

In 2008, we published the first set of guidelines for standardizing research in autophagy.
Since then, this topic has received increasing attention, and many scientists have entered …

Early embryonic development is driven exclusively by maternal gene products deposited
into the oocyte. Although critical in establishing early developmental programs, maternal …

Zebrafish (Danio rerio) are a small‐bodied tropical, freshwater fish species originally from
South Asia. The ease of care, year‐round prolific breeding, and transparent, external …

Inconsistent activity limits the use of CRISPR-Cas9 in zebrafish. We show supernumerary
guanine nucleotides at the 5′ ends of single guide RNAs (sgRNAs) account for diminished …

Neutrophils are the first immune cells recruited to a site of injury or infection, where they
perform many functions. Having completed their role, neutrophils must be removed from the …