Microalgae-based biotechnological sequestration of carbon dioxide for net zero emissions

Z Ma, WY Cheah, IS Ng, JS Chang, M Zhao… - Trends in …, 2022 - cell.com
Excessive carbon dioxide (CO 2) emissions into the atmosphere have become a dire threat
to the human race and environmental sustainability. The ultimate goal of net zero emissions …

Fourth generation biofuel: A review on risks and mitigation strategies

B Abdullah, SAFS Muhammad, Z Shokravi… - … and sustainable energy …, 2019 - Elsevier
Fourth generation biofuel (FGB) uses genetically modified (GM) algae to enhance biofuel
production. Although GM algae biofuel is a well-known alternative to fossil fuels, the …

[HTML][HTML] Emerging technologies in algal biotechnology: toward the establishment of a sustainable, algae-based bioeconomy

M Fabris, RM Abbriano, M Pernice… - Frontiers in plant …, 2020 - frontiersin.org
Mankind has recognized the value of land plants as renewable sources of food, medicine,
and materials for millennia. Throughout human history, agricultural methods were …

Fourth generation biofuel from genetically modified algal biomass: Challenges and future directions

H Shokravi, Z Shokravi, M Heidarrezaei, HC Ong… - Chemosphere, 2021 - Elsevier
Genetic engineering applications in the field of biofuel are rapidly expanding due to their
potential to boost biomass productivity while lowering its cost and enhancing its quality …

Responses and tolerance mechanisms of microalgae to heavy metal stress: A review

X Xiao, W Li, M Jin, L Zhang, L Qin, W Geng - Marine environmental …, 2023 - Elsevier
Microalgae, the primary producers in water ecosystems, are the main food of fish and
shrimp. Microalgae have a great capacity to absorb heavy metals, and low concentrations of …

Cas9 versus Cas12a/Cpf1: Structure–function comparisons and implications for genome editing

DC Swarts, M Jinek - Wiley Interdisciplinary Reviews: RNA, 2018 - Wiley Online Library
Cas9 and Cas12a are multidomain CRISPR‐associated nucleases that can be programmed
with a guide RNA to bind and cleave complementary DNA targets. The guide RNA …

Exploiting endogenous CRISPR-Cas system for multiplex genome editing in Clostridium tyrobutyricum and engineer the strain for high-level butanol production

J Zhang, W Zong, W Hong, ZT Zhang, Y Wang - Metabolic engineering, 2018 - Elsevier
Abstract Although CRISPR-Cas9/Cpf1 have been employed as powerful genome
engineering tools, heterologous CRISPR-Cas9/Cpf1 are often difficult to introduce into …

[HTML][HTML] New applications of synthetic biology tools for cyanobacterial metabolic engineering

M Santos-Merino, AK Singh, DC Ducat - Frontiers in bioengineering …, 2019 - frontiersin.org
Cyanobacteria are promising microorganisms for sustainable biotechnologies, yet unlocking
their potential requires radical re-engineering and application of cutting-edge synthetic …

CO2 bio-mitigation using genetically modified algae and biofuel production towards a carbon net-zero society

A Saravanan, VC Deivayanai, PS Kumar… - Bioresource …, 2022 - Elsevier
CO 2 sequestration carried by biological methodologies shows enhanced potential and has
the advantage that biomass produced from the captured CO 2 can be used for different …

[HTML][HTML] Multiplex gene regulation by CRISPR-ddCpf1

X Zhang, J Wang, Q Cheng, X Zheng, G Zhao, J Wang - Cell discovery, 2017 - nature.com
The clustered regularly interspaced short palindromic repeats (CRISPR)/dCas9 system has
been widely applied in both transcriptional regulation and epigenetic studies. However, for …