DNA double strand breaks need to be repaired in an organized fashion to preserve genomic
integrity. In the organization of faithful repair, histone ubiquitination plays a crucial role …

Dot1/DOT1L catalyzes the methylation of histone H3 lysine 79 (H3K79), which regulates
diverse cellular processes, such as development, reprogramming, differentiation, and …

Oncogene-induced senescence (OIS) is a stable cell cycle arrest that occurs in normal cells
upon oncogene activation. Cells undergoing OIS express a wide variety of secreted factors …

We have developed a targeted method to quantify all combinations of methylation on an H3
peptide containing lysines 27 and 36 (H3K27-K36). By using stable isotopes that separately …

Histone modifications regulate key processes of eukaryotic genomes. Misregulation of the
enzymes that place these modifications can lead to disease. An example of this is DOT1L …

Genetic, biochemical, and cellular studies have uncovered many of the molecular
mechanisms underlying the signaling and repair of chromosomal DNA breaks. However …

Aging induces myriad cellular and, ultimately, physiological changes that cause a decline in
an organism's functional capabilities. Although the aging process and the pathways that …

The methyltransferase DOT1L methylates histone H3 at K79 to facilitate specific biological
events. H3K79 dimethylation (H3K79‐2Me) by DOT1L influences the DNA damage …

Radiotherapy is a major cancer treatment option but dose-limiting side effects such as late-
onset fibrosis in the irradiated tissue severely impair quality of life in cancer survivors. Efforts …

Dietary choline is an important modulator of gene expression (via epigenetic marks) and of
DNA integrity. Choline was discovered to be an essential nutrient for some humans …