Determining how chromosomes are positioned and folded within the nucleus is critical to
understanding the role of chromatin topology in gene regulation. Several methods are …

Abstract The 4D Nucleome Network aims to develop and apply approaches to map the
structure and dynamics of the human and mouse genomes in space and time with the goal …

Using integrative genomic analysis of 360 metastatic castration-resistant prostate cancer
(mCRPC) samples, we identified a novel subtype of prostate cancer typified by biallelic loss …

Recent efforts have succeeded in surveying open chromatin at the single-cell level, but high-
throughput, single-cell assessment of heterochromatin and its underlying genomic …

Totipotency emerges in early embryogenesis, but its molecular underpinnings remain poorly
characterized. In the present study, we employed DNA fiber analysis to investigate how …

The 3D organization of mammalian chromatin was described more than 30 years ago by
visualizing sites of DNA synthesis at different times during the S phase of the cell cycle …

Eukaryotic chromosomes replicate in a temporal order known as the replication-timing
program. In mammals, replication timing is cell-type-specific with at least half the genome …

DNA replication begins with the assembly of pre-replication complexes (pre-RCs) at
thousands of DNA replication origins during the G1 phase of the cell cycle. At the G1–S …

Single-cell epigenome sequencing techniques have recently been developed. However, the
combination of different layers of epigenome sequencing in an individual cell has not yet …

Animal development depends on not only the linear genome sequence that embeds millions
of cis-regulatory elements, but also the three-dimensional (3D) chromatin architecture that …