RecQ helicases are an important family of genome surveillance proteins conserved from
bacteria to humans. Each of the five human RecQ helicases plays critical roles in genome …

Werner syndrome (WS) is a prototypical segmental progeroid syndrome characterized by
multiple features consistent with accelerated aging. It is caused by null mutations of the …

In genome editing with CRISPR–Cas9, transgene integration often remains challenging.
Here, we present an approach for increasing the efficiency of transgene integration by …

Metabolic dysfunction is a primary feature of Werner syndrome (WS), a human premature
aging disease caused by mutations in the gene encoding the Werner (WRN) DNA helicase …

Non-homologous DNA end-joining (NHEJ)—the main pathway for repairing double-
stranded DNA breaks—functions throughout the cell cycle to repair such lesions. Defects in …

Accurate repair of DNA double-strand breaks is essential to life. Indeed, defective DNA
double-strand break repair can lead to toxicity and large scale sequence rearrangements …

The 5′-3′ resection of DNA ends is a prerequisite for the repair of DNA double strand
breaks by homologous recombination, microhomology-mediated end joining, and single …

The double-strand break (DSB) is believed to be one of the most severe types of DNA
damage, and if left unrepaired is lethal to the cell. Several different types of repair act on the …

Very few gene conversions in mitotic cells are associated with crossovers, suggesting that
these events are regulated. This may be important for the maintenance of genetic stability …

Homologous recombination is a ubiquitous process with key functions in meiotic and
vegetative cells for the repair of DNA breaks. It is initiated by the formation of single-stranded …