DNA sequencing, starting with Sanger's chain termination method in 1977 and evolving into
the next generation sequencing (NGS) techniques of today that employ massively parallel …

With the continuous development of massively parallel sequencing (MPS), increasing
numbers of laboratories have utilized this method for forensic genomic analyses. When …

PCR artifacts are an ever-present challenge in sequencing applications. These artifacts can
seriously limit the analysis and interpretation of low-template samples and mixtures …

Stutters are common and well documented artefacts of amplification of short tandem repeat
(STR) regions when using polymerase chain reaction (PCR) occurring as strands one or …

The sequencing of STR markers provides additional information present in the underlying
sequence variation that is typically masked by traditional fragment-based genotyping …

Current approaches for parsing true variation (ie signal) from noise, broadly involve
estimating a baseline value of the latter, below which all sequence data are ignored. In an …

It has been posited that the longest uninterrupted stretch (LUS) of tandem repeats, as
defined by the number of exactly matching repeating motif units, is a better predictor of rates …

Next-generation Sequencing (NGS) is a rapidly evolving technology with demonstrated
benefits for forensic genetic applications, and the strategies to analyze and manage the …

We used a Poisson-gamma model to analyse the allele coverage of autosomal short tandem
repeat (STR) systems obtained by massively parallel sequencing (MPS). The Poisson …

Compound repeats provide a testing ground for evaluating hypotheses on the causes and
consequences of stutter. As compound repeats have two repeating motifs, each motif may …