The accurate and complete replication of genomic DNA is essential for all life. In eukaryotic
cells, the assembly of the multi-enzyme replisomes that perform replication is divided into …

Successful genome duplication is required for cell proliferation and demands extraordinary
precision and accuracy. The mechanisms by which cells enter, progress through, and exit S …

In eukaryotes, DNA replication initiation requires assembly and activation of the
minichromosome maintenance (MCM) 2–7 double hexamer (DH) to melt origin DNA …

The origin recognition complex (ORC) binds sites from which DNA replication is initiated. We
address ORC binding selectivity in vivo by mapping∼ 52,000 ORC2 binding sites …

The human RIF 1 protein controls DNA replication, but the molecular mechanism is largely
unknown. Here, we demonstrate that human RIF 1 negatively regulates DNA replication by …

The chromatin-based rule governing the selection and activation of replication origins in
metazoans remains to be investigated. Here we report that NFIB, a member of Nuclear …

Here, we report a single-cell DNA replication sequencing method, scRepli-seq, a genome-
wide methodology that measures copy number differences between replicated and …

The DNA replication process needs to be coordinated with other DNA metabolism
transactions and must eventually extend to the full genome, regardless of chromatin status …

In metazoan cells, only a limited number of mini chromosome maintenance (MCM)
complexes are fired during S phase, while the majority remain dormant. Several methods …

During routine genome duplication, many potential replication origins remain inactive or
'dormant'. Such origin dormancy is achieved, in part, by an interaction with the metabolic …