ABSTRACT Grapevines (Vitis spp.) in Washington and Oregon were surveyed for the
prevalence of key grapevine viruses. Samples collected from 1,522 vines in Washington …

A reverse transcription (RT) polymerase chain reaction (PCR) assay was developed to allow
rapid, and simultaneous detection of Vitivirus and Foveavirus sequences in two steps. The …

Grapevine rupestris stem pitting-associated virus (GRSPaV), a recently identified virus, is
classified as a member of the Foveavirus genus within the Flexiviridae family. The genome …

Template preparation is important in reverse-transcription polymerase chain reaction (RT-
PCR)-based detection methods. A TissueLyser with tungsten carbide beads was used for …

Grapevine rupestris stem pitting-associated virus (GRSPaV) is a member of the genus
Foveavirus (family Betaflexiviridae, order Tymovirales). GRSPaV was discovered in 1998 …

In this study different instruments and methods used for tissue homogenization, RNA
extraction and quantitative PCR (qPCR) based detection of grapevine RNA viruses were …

Vein necrosis (VN), a virus-like disease latent in all European grapevine cultivars and in
most American rootstock species and hybrids, induces necrosis of the veinlets of its specific …

Grapevine rupestris stem pitting-associated virus (GRSPaV, genus Foveavirus, family
Betaflexiviridae) is one of the most prevalent viruses in grapevines and is associated with …

PCR ELISA is the immunodetection of the products of a polymerase chain reaction (PCR). It
is effective for detecting and differentiating plant viral nucleic acids, but as currently …

Broad spectrum primers were used to amplify a fragment comprising the CP gene and
putative ORF6 by RT-PCR from ds-RNA templates originating from 46 Portuguese varieties …