Many biological processes are executed and regulated through the molecular interactions of
proteins and nucleic acids. Proximity labeling (PL) is a technology for tagging the …

Understanding how chromatin is folded in the nucleus is fundamental to understanding its
function. Although 3D genome organization has been historically difficult to study owing to a …

The epigenome involves a complex set of cellular processes governing genomic activity.
Dissecting this complexity necessitates the development of tools capable of specifically …

This protocol describes the use of TurboID and split-TurboID in proximity labeling
applications for mapping protein–protein interactions and subcellular proteomes in live …

The prokaryote-derived CRISPR–Cas genome editing systems have transformed our ability
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …

Cells compartmentalize their intracellular environment to orchestrate countless
simultaneous biochemical processes. Many intracellular tasks rely on membrane-less …

Proximity labeling catalyzed by promiscuous enzymes, such as TurboID, have enabled the
proteomic analysis of subcellular regions difficult or impossible to access by conventional …

The study of protein subcellular distribution, their assembly into complexes and the set of
proteins with which they interact with is essential to our understanding of fundamental …

Transcription factors (TFs) bind DNA in a sequence-specific manner and thereby serve as
the protein anchors and determinants of 3D genome organization. Conversely, chromatin …

Proximity-based labeling has emerged as a powerful complementary approach to classic
affinity purification of multiprotein complexes in the mapping of protein–protein interactions …