The intestinal tract faces numerous challenges that require several layers of defence. The
tight epithelium forms a physical barrier that is further protected by a mucus layer, which …

Intestinal stem cells at the bottom of crypts fuel the rapid renewal of the different cell types
that constitute a multitasking tissue. The intestinal epithelium facilitates selective uptake of …

INTRODUCTION A reference atlas of human cell types is a major goal for the field. Here, we
set out to generate single-cell atlases of both gene expression (this study) and chromatin …

Spatial barcoding technologies have the potential to reveal histological details of
transcriptomic profiles; however, they are currently limited by their low resolution. Here, we …

Epithelial organoids, such as those derived from stem cells of the intestine, have great
potential for modelling tissue and disease biology,,–. However, the approaches that are …

Current computational workflows for comparative analyses of single-cell datasets typically
use discrete clusters as input when testing for differential abundance among experimental …

Development of the human intestine is not well understood. Here, we link single-cell RNA
sequencing and spatial transcriptomics to characterize intestinal morphogenesis through …

Advanced metastatic cancer poses utmost clinical challenges and may present molecular
and cellular features distinct from an early-stage cancer. Herein, we present single-cell …

The development of single-cell and spatial transcriptomics methods was instrumental in the
conception of the Human Cell Atlas initiative, which aims to generate an integrated map of …

The role of non-neuronal cells in Alzheimer's disease progression has not been fully
elucidated. Using single-nucleus RNA sequencing, we identified a population of disease …