Upon entering a presynaptic terminal, an action potential opens Ca2+ channels, and
transiently increases the local Ca2+ concentration at the presynaptic active zone. Ca2+ then …

SNARE proteins have been proposed to mediate all intracellular membrane fusion events.
There are over 30 SNARE family members in mammalian cells and each is found in a …

phenix. refine is a program within the PHENIX package that supports crystallographic
structure refinement against experimental data with a wide range of upper resolution limits …

Estimates of the quality of experimental maps are important in many stages of structure
determination of macromolecules. Map quality is defined here as the correlation between a …

Plasma membrane wounds are repaired by a mechanism involving Ca 2+-regulated
exocytosis. Elevation in intracellular [Ca 2+] triggers fusion of lysosomes with the plasma …

Membrane fusion can occur between cells, between different intracellular compartments,
between intracellular compartments and the plasma membrane and between lipid-bound …

Secretion of cytolytic granules content at the immunological synapse is a highly regulated
process essential for lymphocyte cytotoxicity. This process requires the rapid transfer of …

The granule exocytosis pathway of cytotoxic lymphocytes is crucial for immune surveillance
and homeostasis. The trafficking of granule components, including the membrane-disruptive …

Regulated exocytosis of secretory granules or dense-core granules has been examined in
many well-characterized cell types including neurons, neuroendocrine, endocrine, exocrine …

Significant time and effort are often required to solve and complete a macromolecular crystal
structure. The development of automated computational methods for the analysis, solution …