The discovery of clustered regularly interspaced short palindromic repeats (CRISPR)
genome editing technology opened the door to provide a versatile approach for treating …

Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …

Eukaryotic cells deploy overlapping repair pathways to resolve DNA damage.
Advancements in genome editing take advantage of these pathways to produce permanent …

Precise gene editing is—or will soon be—in clinical use for several diseases, and more
applications are under development. The programmable nuclease Cas9, directed by a …

Clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9
(Cas9) is a precise genome manipulating technology that can be programmed to induce …

Background Conditional knockout mice and transgenic mice expressing recombinases,
reporters, and inducible transcriptional activators are key for many genetic studies and …

Rapid, efficient generation of knock-in mice with targeted large insertions remains a major
hurdle in mouse genetics. Here, we describe two-cell homologous recombination (2C-HR) …

Investigations of CRISPR gene knockout editing profiles have contributed to enhanced
precision of editing outcomes. However, for homology-directed repair (HDR) in particular …

The use of allogeneic hematopoietic stem cells (HSCs) to treat genetic blood cell diseases
has become a clinical standard but is limited by the availability of suitable matched donors …

In genome editing with CRISPR–Cas9, transgene integration often remains challenging.
Here, we present an approach for increasing the efficiency of transgene integration by …