In-cell NMR: Why and how?
FX Theillet, E Luchinat - Progress in Nuclear Magnetic Resonance …, 2022 - Elsevier
NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as
early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of …
early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of …
NMR spectroscopy for metabolomics in the living system: recent progress and future challenges
Metabolism is a fundamental process that underlies human health and diseases. Nuclear
magnetic resonance (NMR) techniques offer a powerful approach to identify metabolic …
magnetic resonance (NMR) techniques offer a powerful approach to identify metabolic …
In-cell NMR approach for real-time exploration of pathway versatility: substrate mixtures in nonengineered yeast
F Sannelli, PR Jensen, S Meier - Analytical Chemistry, 2023 - ACS Publications
The central carbon metabolism of microbes will likely be used in future sustainable
bioproduction. A sufficiently deep understanding of central metabolism would advance the …
bioproduction. A sufficiently deep understanding of central metabolism would advance the …
Esterase Activity of Serum Albumin Studied by 1H NMR Spectroscopy and Molecular Modelling
DA Belinskaia, PA Voronina, MA Vovk… - International Journal of …, 2021 - mdpi.com
Serum albumin possesses esterase and pseudo-esterase activities towards a number of
endogenous and exogenous substrates, but the mechanism of interaction of various esters …
endogenous and exogenous substrates, but the mechanism of interaction of various esters …
Enhanced 13 C NMR detects extended reaction networks in living cells
PR Jensen, F Sannelli, LT Stauning… - Chemical …, 2021 - pubs.rsc.org
Insights into intracellular chemistry have remained sparse, but would be impactful for the
advancement of biomedicine and bioproduction. A suitable 13C NMR approach provides …
advancement of biomedicine and bioproduction. A suitable 13C NMR approach provides …
Visualizing Metabolism in Biotechnologically Important Yeasts with dDNP NMR Reveals Evolutionary Strategies and Glycolytic Logic
S Meier, KC Wang, F Sannelli, JB Hoof… - Analytical …, 2024 - ACS Publications
Saccharomyces cerevisiae has long been a pillar of biotechnological production and basic
research. More recently, strides to exploit the functional repertoire of nonconventional yeasts …
research. More recently, strides to exploit the functional repertoire of nonconventional yeasts …
Pancreatic β-cells respond to fuel pressure with an early metabolic switch
Pancreatic β-cells become irreversibly damaged by long-term exposure to excessive
glucose concentrations and lose their ability to carry out glucose stimulated insulin secretion …
glucose concentrations and lose their ability to carry out glucose stimulated insulin secretion …
Rapid probing of glucose influx into cancer cell metabolism: using adjuvant and a pH-dependent collection of central metabolites to improve in-cell D-DNP NMR
F Sannelli, KC Wang, PR Jensen, S Meier - Analytical Methods, 2023 - pubs.rsc.org
Changes to metabolism are a hallmark of many diseases. Disease metabolism under
physiological conditions can be probed in real time with in-cell NMR assays. Here, we …
physiological conditions can be probed in real time with in-cell NMR assays. Here, we …
Conversion of Similar Xenochemicals to Dissimilar Products: Exploiting Competing Reactions in Whole-Cell Catalysis
F Sannelli, NC Sindahl, SS Warthegau, PR Jensen… - Molecules, 2023 - mdpi.com
Many enzymes have latent activities that can be used in the conversion of non-natural
reactants for novel organic conversions. A classic example is the conversion of …
reactants for novel organic conversions. A classic example is the conversion of …