Measuring DNA modifications with the comet assay: a compendium of protocols

A Collins, P Møller, G Gajski, S Vodenková… - Nature protocols, 2023 - nature.com
The comet assay is a versatile method to detect nuclear DNA damage in individual
eukaryotic cells, from yeast to human. The types of damage detected encompass DNA …

The accelerated aging phenotype: the role of race and social determinants of health on aging

NN Hooten, NL Pacheco, JT Smith, MK Evans - Ageing Research Reviews, 2022 - Elsevier
The pursuit to discover the fundamental biology and mechanisms of aging within the context
of the physical and social environment is critical to designing interventions to prevent and …

[HTML][HTML] Worldwide research trends in landslide science

P Carrión-Mero, N Montalván-Burbano… - International journal of …, 2021 - mdpi.com
Landslides are generated by natural causes and by human action, causing various
geomorphological changes as well as physical and socioeconomic loss of the environment …

Using the comet and micronucleus assays for genotoxicity studies: A review

RP Araldi, TC de Melo, TB Mendes… - Biomedicine & …, 2015 - Elsevier
Physical, chemical and biological agents can act in the DNA, resulting in mutation involved
in cancer. Thus, genotoxic tests are required by regulatory agencies in order to evaluate …

An overview of comet assay application for detecting DNA damage in aquatic animals

N Jiang, S Naz, Y Ma, Q Ullah, MZ Khan, J Wang, X Lu… - Agriculture, 2023 - mdpi.com
This review discusses several research studies that employed comet assay to evaluate the
environmental impact of genotoxins in aquatic environments. It focuses on in vivo and in situ …

The enzyme-modified comet assay: Past, present and future

D Muruzabal, A Collins, A Azqueta - Food and Chemical Toxicology, 2021 - Elsevier
Highlights•Twelve different enzyme have been used in combination with the comet
assay.•The enzyme-modified comet assay detects several DNA lesions.•Fpg, followed by …

The comet assay: past, present, and future

SAS Langie, A Azqueta, AR Collins - Frontiers in genetics, 2015 - frontiersin.org
The alkaline comet assay (single cell gel electrophoresis) is the most widely used method
for measuring DNA damage in eukaryotic cells (Neri et al., 2015). It detects strand breaks …

[HTML][HTML] The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and …

M Milić, M Ceppi, M Bruzzone, A Azqueta… - … Research/Reviews in …, 2021 - Elsevier
The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular
methods for assessing DNA damage in human population. One of the open issues …

The comet assay: ready for 30 more years

P Møller - Mutagenesis, 2018 - academic.oup.com
During the last 30 years, the comet assay has become widely used for the measurement of
DNA damage and repair in cells and tissues. A landmark achievement was reached in 2016 …

The next three decades of the comet assay: a report of the 11th International Comet Assay Workshop

G Koppen, A Azqueta, B Pourrut, G Brunborg… - …, 2017 - academic.oup.com
Abstract The International Comet Assay Workshops are a series of scientific conferences
dealing with practical and theoretical aspects of the Comet Assay (single-cell gel …