Proteins adopt different higher-order structures (HOS) to enable their unique biological
functions. Understanding the complexities of protein higher-order structures and dynamics …

Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful biophysical
technique being increasingly applied to a wide variety of problems. As the HDX-MS …

Here, we review the current state of the art in analytical and structural methods used to
characterize therapeutic monoclonal antibodies (mAbs) and derivatives. First, a general …

Hydrogen/deuterium exchange (HDX) mass spectrometry (MS) has become a key technique
for monitoring structural and dynamic aspects of proteins in solution. This approach relies on …

Biologics such as monoclonal antibodies are much more complex than small-molecule
drugs, which raises challenging questions for the development and regulatory evaluation of …

The function, efficacy, and safety of protein biopharmaceuticals are tied to their three-
dimensional structure. The analysis and verification of this higher-order structure are critical …

Mass spectrometry-based methods have become increasingly important in structural biology—
in particular for large and dynamic, even heterogeneous assemblies of biomolecules. Native …

Recent technological advances hydrogen exchange MS have led to improvements in the
technique's ability to analyze the shape and movements of proteins. John Engen of …

Over the past two decades, mass spectrometry (MS) has emerged as a bone fide approach
for structural biology. MS can inform on all levels of protein organization, and enables …

Introduction: Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful
methodology to study protein dynamics, protein folding, protein-protein interactions, and …