The growing biopharmaceutical industry has reached a level of maturity that allows for the
monitoring of numerous key variables for both process characterization and outcome …

Ensuring consistent high yields and product quality are key challenges in biomanufacturing.
Even minor deviations in critical process parameters (CPPs) such as media and feed …

Abstract In mammalian cells,> 25% of synthesized proteins are exported through the
secretory pathway. The pathway complexity, however, obfuscates its impact on the secretion …

Highlights•3 waves of innovation have enhanced protein production in CHO cells.•Systems-
level methods are now unravelling drivers of protein production.•Predictive models will …

In this work, we aim to introduce the concept of the degree of hybridization for cell culture
process modeling. We propose that a family of hybrid models can be created with varying …

Introduction: Hybrid modeling combining First-Principles with machine learning is becoming
a pivotal methodology for Biopharma 4.0 enactment. Chinese Hamster Ovary (CHO) cells …

Chinese hamster ovary (CHO) cells are most prevalently used for producing recombinant
therapeutics in biomanufacturing. Recently, more rational and systems approaches have …

Surface plasmon resonance (SPR)-based optical biosensors offer real-time and label-free
analysis of protein interactions, which has extensively contributed to the discovery and …

While machine learning (ML) has made significant contributions to the biopharmaceutical
field, its applications are still in the early stages in terms of providing direct support for quality …

The N-linked glycosylation pattern is an important quality attribute of therapeutic
glycoproteins. It has been reported by our group and by others that different carbon sources …