Recent advances on the multiplex molecular detection of plant viruses and viroids

V Pallás, JA Sánchez-Navarro, D James - Frontiers in microbiology, 2018 - frontiersin.org
Plant viruses are still one of the main contributors to economic losses in agriculture. It has
been estimated that plant viruses can cause as much as 50 billion euros loss worldwide, per …

Detection of eight respiratory bacterial pathogens based on multiplex real‐time PCR with fluorescence melting curve analysis

L Hu, B Han, Q Tong, H Xiao… - Canadian Journal of …, 2020 - Wiley Online Library
Background and Objective. Acinetobacter baumannii, Escherichia coli, Klebsiella
pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae, Staphylococcus aureus …

Lateral flow immunoassay for rapid detection of grapevine leafroll-associated virus

NA Byzova, SV Vinogradova, EV Porotikova… - Biosensors, 2018 - mdpi.com
Grapevine leafroll-associated virus 3 (GLRaV-3) is one of the main pathogens of grapes,
causing a significant loss in yield and decrease in quality for this agricultural plant. For …

Simultaneous detection of five pig viruses associated with enteric disease in pigs using EvaGreen real-time PCR combined with melting curve analysis

D Wen, G Liu, T Opriessnig, Z Yang, Y Jiang - Journal of virological …, 2019 - Elsevier
In recent years, a series of porcine diarrhea viruses such as porcine epidemic diarrhea virus
(PEDV), transmissible gastroenteritis virus (TGEV), rotaviruses of group A (RVA), rotaviruses …

A Novel and Highly Inclusive Quantitative Real-Time RT-PCR Method for the Broad and Efficient Detection of Grapevine Leafroll-Associated Virus 1

F Morán, A Olmos, M Glasa, MBD Silva, V Maliogka… - Plants, 2023 - mdpi.com
Grapevine (Vitis vinifera L.) is one of the most important crops in the world due to its
economic and social impact. Like many other crops, grapevine is susceptible to different …

Development of a Melting Curve-Based Triple Eva Green Real-Time PCR Assay for Simultaneous Detection of Three Shrimp Pathogens

X Dong, Y Chen, H Lou, G Wang, C Zhou, L Wang, X Li… - Animals, 2024 - mdpi.com
Simple Summary Shrimp aquaculture is an important industry due to its advantages of short
production cycles and good economic benefits, but it faces challenges from diseases …

Affimer reagents as tools in diagnosing plant virus diseases

EL Hesketh, C Tiede, H Adamson, TL Adams… - Scientific reports, 2019 - nature.com
Plant viruses can cause devastating losses to agriculture and are therefore a major threat to
food security. The rapid identification of virally-infected crops allowing containment is …

One-step real-time multiplex reverse transcription-polymerase chain reaction assay with melt curve analysis for detection of potato leafroll virus, potato virus S, potato …

N Onozuka, T Ohki, N Oka, T Maoka - Virology journal, 2021 - Springer
Background Certification of seed potato as free of viruses is essential for stable potato
production. Among more than 30 virus species infecting potato, potato leafroll virus (PLRV) …

A new molecular approach to the diagnosis of small ruminant morbillivirus withEvaGreen based assay

ME Öz, E Torlak, O Avci, M Doğan… - Turkish Journal of …, 2021 - journals.tubitak.gov.tr
The present work describes a new method that is anticipated to contribute to the global
control and eradication strategies of small ruminant morbillivirus (SRMV), which negatively …

Development and validation of RT-PCR multiplex detection of grapevine viruses and viroids in the Czech Republic

M Komínková, P Komínek - Journal of Plant Pathology, 2020 - Springer
Abstract A multiplex RT-PCR reaction was developed and validated for the simultaneous
detection of two viruses and two viroids: grapevine fleck virus, grapevine Pinot gris virus …