Ultrasensitive and highly specific lateral flow assays for point-of-care diagnosis

Y Liu, L Zhan, Z Qin, J Sackrison, JC Bischof - ACS nano, 2021 - ACS Publications
Lateral flow assays (LFAs) are paper-based point-of-care (POC) diagnostic tools that are
widely used because of their low cost, ease of use, and rapid format. Unfortunately …

CRISPR‐Cas biochemistry and CRISPR‐based molecular diagnostics

Z Weng, Z You, J Yang, N Mohammad… - Angewandte Chemie …, 2023 - Wiley Online Library
Polymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in
disease diagnostics, pathogen surveillance, and many more. However, this method requires …

Amplification-free RNA detection with CRISPR–Cas13

H Shinoda, Y Taguchi, R Nakagawa, A Makino… - Communications …, 2021 - nature.com
CRISPR-based nucleic-acid detection is an emerging technology for molecular diagnostics.
However, these methods generally require several hours and could cause amplification …

Single molecule protein detection with attomolar sensitivity using droplet digital enzyme-linked immunosorbent assay

L Cohen, N Cui, Y Cai, PM Garden, X Li, DA Weitz… - ACS …, 2020 - ACS Publications
Many proteins are present at low concentrations in biological samples, and therefore,
techniques for ultrasensitive protein detection are necessary. To overcome challenges with …

Cellphone-based hand-held microplate reader for point-of-care testing of enzyme-linked immunosorbent assays

B Berg, B Cortazar, D Tseng, H Ozkan, S Feng, Q Wei… - ACS …, 2015 - ACS Publications
Standard microplate based enzyme-linked immunosorbent assays (ELISA) are widely
utilized for various nanomedicine, molecular sensing, and disease screening applications …

Ultrasensitive detection of attomolar protein concentrations by dropcast single molecule assays

C Wu, PM Garden, DR Walt - Journal of the American Chemical …, 2020 - ACS Publications
Measurements of very low levels of biomolecules, including proteins and nucleic acids,
remain a critical challenge in many clinical diagnostic applications due to insufficient …

Automated amplification-free digital RNA detection platform for rapid and sensitive SARS-CoV-2 diagnosis

H Shinoda, T Iida, A Makino, M Yoshimura… - Communications …, 2022 - nature.com
In the ongoing COVID-19 pandemic, rapid and sensitive diagnosis of viral infection is a
critical deterrent to the spread of SARS-CoV-2. To this end, we developed an automated …

Advances in Optical Single‐Molecule Detection: En Route to Supersensitive Bioaffinity Assays

Z Farka, MJ Mickert, M Pastucha… - Angewandte Chemie …, 2020 - Wiley Online Library
The ability to detect low concentrations of analytes and in particular low‐abundance
biomarkers is of fundamental importance, eg, for early‐stage disease diagnosis. The …

Ultrarapid generation of femtoliter microfluidic droplets for single-molecule-counting immunoassays

J Shim, RT Ranasinghe, CA Smith, SM Ibrahim… - ACS …, 2013 - ACS Publications
We report a microfluidic droplet-based approach enabling the measurement of chemical
reactions of individual enzyme molecules and its application to a single-molecule-counting …

[图书][B] Smart CMOS image sensors and applications

J Ohta - 2020 - taylorfrancis.com
Revised and expanded for this new edition, Smart CMOS Image Sensors and Applications,
Second Edition is the only book available devoted to smart CMOS image sensors and …