Animal cell culture technology has advanced significantly over the last few decades and is
now generally considered a reliable, robust and relatively mature technology. A range of …

Molecular techniques now allow the design of precise genetic modifications in the mouse.
Not only can defined nucleotide changes be engineered into the genome of the mouse, but …

The gene Xist initiates the chromosomal silencing process of X inactivation in mammals. Its
product, a noncoding RNA, is expressed from and specifically associates with the inactive X …

Mouse models of human disease can be generated by homologous recombination for
germline loss-of-function mutations. However, embryonic-lethal phenotypes and systemic …

The BLAST algorithm was developed as a way to perform DNA and protein sequence
similarity searches by an algorithm that is faster than FASTA but considered to be equally as …

Almost all of the 200 or so approved biopharmaceuticals have been produced in one of
three host systems: the bacterium Escherichia coli, yeasts (Saccharomyces cerevisiae …

Revised Manuscript Received August 31, 1994® abstract: Using the FLP/FRT system for site-
specific recombination and the wild-type recognition site (FRT) in conjunction with certain …

Facioscapulohumeral muscular dystrophy (FSHD) is caused by an unusual deletion with
neomorphic activity. This deletion derepresses genes in cis; however which candidate gene …

The Cre recombinase mediates precise site-specific recombination between a pair of loxP
sequences through an intermediate containing Holiday junction. The recombination junction …

Site-specific recombinases from bacteriophage and yeasts have been developed as novel
tools for manipulating DNA both in the test-tube and in living organisms. We discuss the …