Manipulation of individual molecules with optical tweezers provides a powerful means of
interrogating the structure and folding of proteins. Mechanical force is not only a relevant …

The use of force probes to induce unfolding and refolding of single molecules through the
application of mechanical tension, known as single-molecule force spectroscopy (SMFS) …

The folding/misfolding and pharmacological rescue of multidomain ATP-binding cassette
(ABC) C-subfamily transporters, essential for organismal health, remain incompletely …

Many proteins in the cell fold cotranslationally within the restricted space of the polypeptide
exit tunnel or at the surface of the ribosome. A growing body of evidence suggests that the …

Large biomolecular systems are at the heart of many essential cellular processes. The
dynamics and energetics of an increasing number of these systems are being studied by …

Talin is a force-sensing multidomain protein and a major player in cellular
mechanotransduction. Here, we use single-molecule magnetic tweezers to investigate the …

摘要光镊采用聚焦的激光束束缚微米, 纳米级的粒子, 具有亚皮牛级的力分辨率和亚毫秒级的
时间响应, 在单分子生物物理中具有广泛的应用. 通过化学耦链将生物大分子连接到高分子微球 …

Growing cells invest a significant part of their biosynthetic capacity into the production of
proteins. To become functional, newly-synthesized proteins must be N-terminally processed …

Proteins can begin the conformational search for their native structure in parallel with
biosynthesis on the ribosome, in a process termed co-translational folding. In contrast to the …

αβ T cell receptors (TCRs) principally recognize aberrant peptides bound to major
histocompatibility complex molecules (pMHCs) on unhealthy cells, amplifying specificity and …