Biocatalysis has undergone revolutionary progress in the past century. Benefited by the
integration of multidisciplinary technologies, natural enzymatic reactions are constantly …

Genetically encoded biosensors are the vital components of synthetic biology and metabolic
engineering, as they are regarded as powerful devices for the dynamic control of genotype …

Due to the ability to regulate target metabolic pathways globally and dynamically, metabolic
regulation systems composed of transcription factors have been widely used in metabolic …

Low yield and low titer of natural products are common issues in natural product
biosynthesis through microbial cell factories. One effective way to resolve such bottlenecks …

In vivo biosensors that can convert metabolite concentrations into measurable output signals
are valuable tools for high-throughput screening and dynamic pathway control in the field of …

Highlights•Transcription factor-based biosensors are powerful analytical synthetic biology
tools.•Biosensors can be used for detection, metabolic engineering, and protein …

The reprogramming of metabolism in response to switching the carbon source from glucose
to non-preferred carbon sources is well-studied for yeast. However, understanding how …

Sharomyces cerevisiae is currently one of the most important foreign gene expression
systems. S. cerevisiae is an excellent host for high-value metabolite cell factories due to its …

Transcription factor (TF)-based biosensors are widely used for the detection of metabolites
and the regulation of cellular pathways in response to metabolites. Several challenges …

Metabolic regulation of gene expression for the microbial production of fine chemicals, such
as organic acids, is an important research topic in post-genomic metabolic engineering. In …