Conspectus Non-heme iron dioxygenases catalyze vital processes for human health related
to the biosynthesis of essential products and the biodegradation of toxic metabolites. Often …

What happens inside an enzyme's active site to allow slow and difficult chemical reactions to
occur so rapidly? This question has occupied biochemists' attention for a long time …

Nitriles are widely used vibrational probes; however, the interpretation of their IR
frequencies is complicated by hydrogen bonding (H-bonding) in protic environments. We …

Vibrational probes can provide a direct readout of the local electrostatic field in complex
molecular environments, such as protein binding sites and enzyme active sites. This …

Infrared spectroscopy has played an instrumental role in the study of a wide variety of
biological questions. However, in many cases, it is impossible or difficult to rely on the …

Conspectus Enzymes are essential for all living organisms, and their effectiveness as
chemical catalysts has driven more than a half century of research seeking to understand …

Vibrational spectroscopy provides a direct route to the physicochemical characterization of
molecules. While both IR and Raman spectroscopy have been used for decades to provide …

Enzymes use protein architectures to create highly specialized structural motifs that can
greatly enhance the rates of complex chemical transformations. Here, we use experiments …

Understanding how applied potentials and electrolyte solution conditions affect interfacial
proton (charge) transfers at electrode surfaces is critical for electrochemical technologies …

We report the effect of conformational dynamics on the fluctuations of electric fields in the
active site of the enzyme ketosteroid isomerase (KSI). While KSI is considered to be a rigid …