Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives

L Baldi, DL Hacker, M Adam, FM Wurm - Biotechnology letters, 2007 - Springer
The expansion of the biologics pipeline depends on the identification of candidate proteins
for clinical trials. Speed is one of the critical issues, and the rapid production of high quality …

Large-scale transfection of mammalian cells for the fast production of recombinant protein

PL Pham, A Kamen, Y Durocher - Molecular biotechnology, 2006 - Springer
Recombinant proteins (r-proteins) are increasingly important in fundamental research and
for clinical applications. As many of these r-proteins are of human or animal origin, cultivated …

A versatile ligation-independent cloning method suitable for high-throughput expression screening applications

NS Berrow, D Alderton, S Sainsbury… - Nucleic acids …, 2007 - academic.oup.com
This article describes the construction of a set of versatile expression vectors based on the In-
Fusion™ cloning enzyme and their use for high-throughput cloning and expression …

Mechanistic model for production of recombinant adeno-associated virus via triple transfection of HEK293 cells

TNT Nguyen, S Sha, MS Hong, AJ Maloney… - … Therapy Methods & …, 2021 - cell.com
Manufacturing of recombinant adeno-associated virus (rAAV) viral vectors remains
challenging, with low yields and low full: empty capsid ratios in the harvest. To elucidate the …

Creation of a high-yield AAV vector production platform in suspension cells using a design-of-experiment approach

H Zhao, KJ Lee, M Daris, Y Lin, T Wolfe, J Sheng… - … Therapy Methods & …, 2020 - cell.com
Recombinant adeno-associated virus (rAAV) vectors are a leading gene delivery platform,
but vector manufacturing remains a challenge. New methods are needed to increase rAAV …

Applications and analysis of hydrolysates in animal cell culture

YY Ho, HK Lu, ZFS Lim, HW Lim, YS Ho… - Bioresources and …, 2021 - Springer
Animal cells are used in the manufacturing of complex biotherapeutic products since the
1980s. From its initial uses in biological research to its current importance in the …

Insertion of the designed helical linker led to increased expression of tf-based fusion proteins

N Amet, HF Lee, WC Shen - Pharmaceutical research, 2009 - Springer
Purpose To demonstrate a high-level expression of transferrin (Tf)-based fusion proteins by
inserting a helical linker between two protein domains. Methods Tf-based fusion proteins …

Development of a scalable process for high‐yield lentiviral vector production by transient transfection of HEK293 suspension cultures

S Ansorge, S Lanthier, J Transfiguracion… - The Journal of Gene …, 2009 - Wiley Online Library
Background Lentiviral vectors (LV) offer several advantages over other gene delivery
vectors. Their potential for the integration and long‐term expression of therapeutic genes …

Advancements in mammalian cell transient gene expression (TGE) technology for accelerated production of biologics

S Gutiérrez-Granados, L Cervera… - Critical reviews in …, 2018 - Taylor & Francis
Transient gene expression (TGE) in animal cell cultures has been used for almost 30 years
to produce milligrams and grams of recombinant proteins, virus-like particles and viral …

High‐density transfection with HEK‐293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI

G Backliwal, M Hildinger, V Hasija… - Biotechnology and …, 2008 - Wiley Online Library
Recombinant proteins are of great commercial and scientific interest. Yet, most production
methods in mammalian cells involve the time‐and labor‐consuming step of creating stable …