In vivo two-photon calcium imaging (2PCI) is a technique used for recording neuronal
activity in the intact brain. It is based on the principle that, when neurons fire action …

Calcium ions generate versatile intracellular signals that control key functions in all types of
neurons. Imaging calcium in neurons is particularly important because calcium signals exert …

High-resolution optical imaging is critical to understanding brain function. We demonstrate
that three-photon microscopy at 1,300-nm excitation enables functional imaging of …

Neuronal representations change as associations are learned between sensory stimuli and
behavioral actions. However, it is poorly understood whether representations for learned …

Imaging is used to map activity across populations of neurons. Microscopes with cellular
resolution have small (< 1 millimeter) fields of view and cannot simultaneously image activity …

Light scattering was thought to be the fundamental limitation for the depth at which optical
imaging methods can retain their resolution and sensitivity. However, it was shown that light …

Heavy metal ions are of great concern, not only among the scientific community, especially
chemists, biologists, and environmentalists, but increasingly among the general population …

Multiphoton microscopy has enabled unprecedented dynamic exploration in living
organisms. A significant challenge in biological research is the dynamic imaging of features …

Here we describe an automated method, named serial two-photon (STP) tomography, that
achieves high-throughput fluorescence imaging of mouse brains by integrating two-photon …

Linking neural microcircuit function to emergent properties of the mammalian brain requires
fine-scale manipulation and measurement of neural activity during behavior, where each …