Organisms from all domains of life invest a substantial amount of energy for the introduction
of RNA modifications into nearly all transcripts studied to date. Instrumental analysis of RNA …

The chemistry of guide RNA (gRNA) affects the performance of the clustered regularly
interspaced short palindromic repeats (CRISPR)-Cas9 genome editing technique. However …

The orthogonality of separation between ion-pair reversed phase (IP-RP), anion exchange
(AEX), and hydrophilic interaction liquid chromatography (HILIC) was evaluated for …

Small interfering RNA (siRNA), consisting of two complementary single-stranded RNAs with
overhanging bases, is being adopted as a potent and specific inhibitor of target gene …

Therapeutic oligonucleotides (ONs) are typically manufactured via solid-phase synthesis,
characterized by limited scalability and huge environmental footprint, limiting their …

RNA modifications gain growing attention as a new frontier in the life sciences but with the
rise of RNA vaccines also in biomedical drug development. Impeccable characterization of …

The main goal of this study was the investigation of the impact of several ionic liquids,
commonly used as free silanol suppressors, on the retention and separation of …

Abstract Riassunto tesi dottorato, Lorenzo Preti Questo lavoro di tesi si concentra sulla
bioconiugazione, riflettendo sugli argomenti affrontati durante i miei studi di dottorato. La tesi …

The initial goal of the project was to develop a more effective method for the purifications of
oligonucleotides. This was achieved by developing a method of ion-exchange based on …

Aptamers, single stranded RNA or DNA that bind to target ligands with high affinity and
specificity, have a wide range of applications, from therapeutics to biosensing, which makes …