Efficiency correction is required for accurate quantitative PCR analysis and reporting

JM Ruijter, RJ Barnewall, IB Marsh… - Clinical …, 2021 - academic.oup.com
Abstract Background Quantitative PCR (qPCR) aims to measure the DNA or RNA
concentration in diagnostic and biological samples based on the quantification cycle (Cq) …

[HTML][HTML] A survey of tools for the analysis of quantitative PCR (qPCR) data

S Pabinger, S Rödiger, A Kriegner, K Vierlinger… - Biomolecular Detection …, 2014 - Elsevier
Real-time quantitative polymerase-chain-reaction (qPCR) is a standard technique in most
laboratories used for various applications in basic research. Analysis of qPCR data is a …

[HTML][HTML] Co-quantification of crAssphage increases confidence in wastewater-based epidemiology for SARS-CoV-2 in low prevalence areas

ML Wilder, F Middleton, DA Larsen, Q Du, A Fenty… - Water research X, 2021 - Elsevier
Wastewater surveillance of SARS-CoV-2 RNA is increasingly being incorporated into public
health efforts to respond to the COVID-19 pandemic. In order to obtain the maximum benefit …

[HTML][HTML] An evaluation of R2 as an inadequate measure for nonlinear models in pharmacological and biochemical research: a Monte Carlo approach

AN Spiess, N Neumeyer - BMC pharmacology, 2010 - Springer
Background It is long known within the mathematical literature that the coefficient of
determination R 2 is an inadequate measure for the goodness of fit in nonlinear models …

Rapid detection of SARS-CoV-2 by low volume real-time single tube reverse transcription recombinase polymerase amplification using an exo probe with an internally …

O Behrmann, I Bachmann, M Spiegel… - Clinical …, 2020 - academic.oup.com
Background The current outbreak of SARS-CoV-2 has spread to almost every country with
more than 5 million confirmed cases and over 300,000 deaths as of May 26, 2020. Rapid …

Why the need for qPCR publication guidelines?—The case for MIQE

SA Bustin - Methods, 2010 - Elsevier
The polymerase chain reaction (PCR) has matured from a labour-and time-intensive, low
throughput qualitative gel-based technique to an easily automated, rapid, high throughput …

Simple absolute quantification method correcting for quantitative PCR efficiency variations for microbial community samples

R Brankatschk, N Bodenhausen, J Zeyer… - Applied and …, 2012 - Am Soc Microbiol
Real-time quantitative PCR (qPCR) is a widely used technique in microbial community
analysis, allowing the quantification of the number of target genes in a community sample …

[HTML][HTML] Quantification of colorimetric isothermal amplification on the smartphone and its open-source app for point-of-care pathogen detection

HQ Nguyen, VD Nguyen, H Van Nguyen, TS Seo - Scientific Reports, 2020 - nature.com
The increasing risk of infectious pathogens, especially in the under-developed countries, is
demanding the development of point-of-care (POC) nucleic acid testing in the low-resource …

Benefits of higher resistance‐training volume are related to ribosome biogenesis

D Hammarström, S Øfsteng, L Koll… - The Journal of …, 2020 - Wiley Online Library
Key points For individuals showing suboptimal adaptations to resistance training,
manipulation of training volume is a potential measure to facilitate responses. This remains …

[HTML][HTML] Independent origin of large labyrinth size in turtles

SW Evers, WG Joyce, JN Choiniere, GS Ferreira… - Nature …, 2022 - nature.com
The labyrinth of the vertebrate inner ear is a sensory system that governs the perception of
head rotations. Central hypotheses predict that labyrinth shape and size are related to …