Muscle stem cell isolation and in vitro culture for meat production: A methodological review

KH Choi, JW Yoon, M Kim, HJ Lee… - … reviews in food …, 2021 - Wiley Online Library
Cultured muscle tissue‐based protein products, also known as cultured meat, are produced
through in vitro myogenesis involving muscle stem cell culture and differentiation, and …

Biomedical applications of electrical stimulation

S Zhao, AS Mehta, M Zhao - Cellular and molecular life sciences, 2020 - Springer
This review provides a comprehensive overview on the biomedical applications of electrical
stimulation (EStim). EStim has a wide range of direct effects on both biomolecules and cells …

Formation of contractile 3D bovine muscle tissue for construction of millimetre-thick cultured steak

M Furuhashi, Y Morimoto, A Shima, F Nakamura… - npj Science of …, 2021 - nature.com
Owing to the increase in the global demand of meat, cultured meat technology is being
developed to circumvent a shortage of meat in the future. However, methods for construction …

[HTML][HTML] Microfluidic-enhanced 3D bioprinting of aligned myoblast-laden hydrogels leads to functionally organized myofibers in vitro and in vivo

M Costantini, S Testa, P Mozetic, A Barbetta, C Fuoco… - Biomaterials, 2017 - Elsevier
We present a new strategy for the fabrication of artificial skeletal muscle tissue with
functional morphologies based on an innovative 3D bioprinting approach. The methodology …

Electrical stimulation increases hypertrophy and metabolic flux in tissue-engineered human skeletal muscle

A Khodabukus, L Madden, NK Prabhu, TR Koves… - Biomaterials, 2019 - Elsevier
In vitro models of contractile human skeletal muscle hold promise for use in disease
modeling and drug development, but exhibit immature properties compared to native adult …

Engineering biomimetic materials for skeletal muscle repair and regeneration

KH Nakayama, M Shayan… - Advanced healthcare …, 2019 - Wiley Online Library
Although skeletal muscle is highly regenerative following injury or disease, endogenous self‐
regeneration is severely impaired in conditions of volume traumatic muscle loss …

Prolonged culture of aligned skeletal myotubes on micromolded gelatin hydrogels

A Bettadapur, GC Suh, NA Geisse, ER Wang, C Hua… - Scientific reports, 2016 - nature.com
In vitro models of skeletal muscle are critically needed to elucidate disease mechanisms,
identify therapeutic targets and test drugs pre-clinically. However, culturing skeletal muscle …

Tissue-engineered skeletal muscle models to study muscle function, plasticity, and disease

A Khodabukus - Frontiers in Physiology, 2021 - frontiersin.org
Skeletal muscle possesses remarkable plasticity that permits functional adaptations to a
wide range of signals such as motor input, exercise, and disease. Small animal models have …

Electrical pulse stimulation of cultured skeletal muscle cells as a model for in vitro exercise – possibilities and limitations

N Nikolić, SW Görgens, GH Thoresen, V Aas… - Acta …, 2017 - Wiley Online Library
The beneficial health‐related effects of exercise are well recognized, and numerous studies
have investigated underlying mechanism using various in vivo and in vitro models. Although …

Regenerative medicine for skeletal muscle loss: A review of current tissue engineering approaches

B Langridge, M Griffin, PE Butler - Journal of Materials Science: Materials …, 2021 - Springer
Skeletal muscle is capable of regeneration following minor damage, more significant
volumetric muscle loss (VML) however results in permanent functional impairment. Current …