Protein glycosylation involves the co-translational or post-translational addition of glycans to
proteins and is a crucial protein modification in health and disease. The aim of …

Introduction: Western blotting is one of the most commonly used techniques in molecular
biology and proteomics. Since western blotting is a multistep protocol, variations and errors …

Efficient and reproducible sample preparation is a prerequisite for any robust and sensitive
quantitative bottom-up proteomics workflow. Here, we performed an independent …

Robust, efficient, and reproducible protein extraction and sample processing is a key step for
bottom-up proteomics analyses. While many sample preparation protocols for mass …

Single cell proteomic analysis provides crucial information on cellular heterogeneity in
biological systems. Herein, we describe a nanoliter-scale oil-air-droplet (OAD) chip for …

Bottom-up proteomic strategies rely on efficient digestion of proteins into peptides for mass
spectrometry analysis. In-solution and filter-based strategies are commonly used for …

The multiplexing capabilities of isobaric mass tag-based protein quantification, such as
Tandem Mass Tags or Isobaric Tag for Relative and Absolute Quantitation have dramatically …

Plant phosphoproteomics provides a global view of phosphorylation-mediated signaling in
plants; however, it demands high-throughput methods with sensitive detection and accurate …

Bacteria are the most abundant and diverse organisms among the kingdoms of life. Due to
this excessive variance, finding a unified, comprehensive, and safe workflow for quantitative …

Metaproteomics is a promising methodology for the functional characterizations of the gut
microbiome. However, the performance of metaproteomic analysis is affected by protein …