Why formalin-fixed, paraffin-embedded biospecimens must be used in genomic medicine: an evidence-based review and conclusion
W Mathieson, GA Thomas - Journal of Histochemistry & …, 2020 - journals.sagepub.com
Fresh-frozen tissue is the “gold standard” biospecimen type for next-generation sequencing
(NGS). However, collecting frozen tissue is usually not feasible because clinical workflows …
(NGS). However, collecting frozen tissue is usually not feasible because clinical workflows …
Major p53 immunohistochemical patterns in in situ and invasive squamous cell carcinomas of the vulva and correlation with TP53 mutation status
B Tessier-Cloutier, KE Kortekaas, E Thompson… - Modern …, 2020 - nature.com
The recent literature has shown that vulvar squamous cell carcinoma (VSCC) can be
stratified into two prognostically relevant groups based on human papillomavirus (HPV) …
stratified into two prognostically relevant groups based on human papillomavirus (HPV) …
[HTML][HTML] FFPE-based NGS approaches into clinical practice: the limits of glory from a pathologist viewpoint
F Cappello, V Angerilli, G Munari, C Ceccon… - Journal of Personalized …, 2022 - mdpi.com
The introduction of next-generation sequencing (NGS) in the molecular diagnostic
armamentarium is deeply changing pathology practice and laboratory frameworks. NGS …
armamentarium is deeply changing pathology practice and laboratory frameworks. NGS …
[HTML][HTML] The mutational signatures of formalin fixation on the human genome
Clinical archives of patient material near-exclusively consist of formalin-fixed and paraffin-
embedded (FFPE) blocks. The ability to precisely characterise mutational signatures from …
embedded (FFPE) blocks. The ability to precisely characterise mutational signatures from …
[HTML][HTML] Use of FFPE-derived DNA in next generation sequencing: DNA extraction methods
Archival tissues represent a rich resource for clinical genomic studies, particularly when
coupled with comprehensive medical records. Use of these in next generation sequencing …
coupled with comprehensive medical records. Use of these in next generation sequencing …
[HTML][HTML] Molecular characterization of invasive and in situ squamous neoplasia of the vulva and implications for morphologic diagnosis and outcome
B Tessier-Cloutier, J Pors, E Thompson, J Ho… - Modern Pathology, 2021 - Elsevier
Human papillomavirus (HPV)-independent vulvar squamous cell carcinoma (VSCC) is an
aggressive clinical entity. Current diagnostic guidelines for premalignant lesions are …
aggressive clinical entity. Current diagnostic guidelines for premalignant lesions are …
Comprehensive profiling identifies tumour and immune microenvironmental differences in clinical subsets of cutaneous squamous cell carcinoma
Background Cutaneous squamous cell carcinomas (cSCCs) are the second most diagnosed
skin cancer worldwide; however, little is known about the pathobiological factors that …
skin cancer worldwide; however, little is known about the pathobiological factors that …
[HTML][HTML] p53-Abnormal “Fields of dysplasia” in human papillomavirus–independent vulvar squamous cell carcinoma impacts margins and recurrence risk
EF Thompson, RWC Wong, G Trevisan… - Modern Pathology, 2023 - Elsevier
Abstract Abnormal p53 (p53abn) immunohistochemical (IHC) staining patterns can be found
in vulvar squamous cell carcinoma (VSCC) and differentiated vulvar intraepithelial …
in vulvar squamous cell carcinoma (VSCC) and differentiated vulvar intraepithelial …
[HTML][HTML] Evaluation of the use of formalin-fixed and paraffin-embedded archive gastric tissues for microbiota characterization using next-generation sequencing
I Pinto-Ribeiro, RM Ferreira, J Pereira-Marques… - International Journal of …, 2020 - mdpi.com
Large numbers of well-characterized clinical samples are fundamental to establish relevant
associations between the microbiota and disease. Formalin-fixed and paraffin-embedded …
associations between the microbiota and disease. Formalin-fixed and paraffin-embedded …
[HTML][HTML] LYmphoid NeXt-Generation Sequencing (LYNX) panel: a comprehensive capture-based sequencing tool for the analysis of prognostic and predictive markers …
V Navrkalova, K Plevova, J Hynst, K Pal… - The Journal of Molecular …, 2021 - Elsevier
B-cell neoplasms represent a clinically heterogeneous group of hematologic malignancies
with considerably diverse genomic architecture recently endorsed by next-generation …
with considerably diverse genomic architecture recently endorsed by next-generation …