Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Protein subcellular localization is tightly controlled and intimately linked to protein function in
health and disease. Capturing the spatial proteome—that is, the localizations of proteins and …

Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era.
We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data …

We introduce APEX-seq, a method for RNA sequencing based on direct proximity labeling of
RNA using the peroxidase enzyme APEX2. APEX-seq in nine distinct subcellular locales …

Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and
cellular structures at the nanometer scale. Employing these techniques in living cells has …

Mitochondria are inextricably linked to the development of diseases and cell metabolism
disorders. Super-resolution imaging (SRI) is crucial in enhancing our understanding of …

INTRODUCTION Our textbook understanding of the nanoscale organization of the cell and
its relationship to the thousands of proteins that drive cellular metabolism comes largely from …

Super-resolved structured illumination microscopy (SR-SIM) is among the most rapidly
growing fluorescence microscopy techniques that can surpass the optical diffraction limit …

Next-generation sequencing has provided a more complete picture of the composition of the
human transcriptome indicating that much of the “blueprint” is a vastness of poorly …

INTRODUCTION Neural circuits across the brain are composed of structures spanning
seven orders of magnitude in size that are assembled from thousands of distinct protein …