Structures of two restriction endonucleases, BamHI and PvuII, were reported in the past
year. This doubles the number of restriction endonuclease structures now known from two to …

The catalytic mechanisms of type II restriction endonucleases and homing endonucleases
are discussed and compared. Brief reviews of the chemistry of phosphoryl transfers and …

Publisher Summary This chapter discusses phase determination from multiwavelength
anomalous diffraction (MAD) measurements. The MAD approach to macromolecular …

The crystal structure of restriction endonuclease Bam HI complexed to DNA has been
determined at 2.2 angstrom resolution. The DNA binds in the cleft and retains a B-DNA type …

FokI is a bipartite restriction endonuclease that recognizes a non-palindromic DNA
sequence, and then makes double-stranded cuts outside of that sequence to leave a 5 …

Type II restriction enzymes are characterized by their remarkable specificity and simplicity.
They require only divalent metals (such as Mg 2+ or Mn 2+) as cofactors to catalyze the …

We have determined the structure of PvuII endonuclease complexed with cognate DNA by X‐
ray crystallography. The DNA substrate is bound with a single homodimeric protein, each …

Background: Elicitins form a novel class of plant necrotic proteins which are secreted by
Phytophthora and Pythium fungi, parasites of many economically important crops. These …

The alternative splicing of mRNA is a critical process in higher eukaryotes that generates
substantial proteomic diversity. Many of the proteins that are essential to this process contain …

Restriction endonucleases are remarkably resilient to alterations in their DNA binding
specificity. To understand the basis of this immutability, we have determined the crystal …