We reviewed the current methodology and practices of the DNA metabarcoding approach
using a universal PCR primer pair MiFish, which co-amplifies a short fragment of fish DNA …

Vast global declines of freshwater and marine fish diversity and population abundance pose
serious threats to both ecosystem sustainability and human livelihoods. Environmental DNA …

Environmental DNA (eDNA) refers to DNA that can be extracted from environmental
samples (such as soil, water, feces, or air) without the prior isolation of any target organism …

Global biodiversity in freshwater and the oceans is declining at high rates. Reliable tools for
assessing and monitoring aquatic biodiversity, especially for rare and secretive species, are …

Community-level data, the type generated by an increasing number of metabarcoding
studies, is often graphed as stacked bar charts or pie graphs that use color to represent taxa …

Metabarcoding extra‐organismal DNA from environmental samples is now a key technique
in aquatic biomonitoring and ecosystem health assessment. Of critical consideration when …

DNA metabarcoding offers new perspectives in biodiversity research. This recently
developed approach to ecosystem study relies heavily on the use of next‐generation …

The field of environmental DNA (eDNA) analysis has rapidly developed over the past
decade and the technique has become widely used for detecting aquatic macroorganisms in …

Knowledge of species distribution is critical to ecological management and conservation
biology. Effective management requires the detection of populations, which can sometimes …

In the era of “Anthropocene defaunation,” large species are often no longer detected in
habitats where they formerly occurred. However, it is unclear whether this apparent missing …