[HTML][HTML] Strategies for immortalization of primary hepatocytes

E Ramboer, B De Craene, J De Kock, T Vanhaecke… - Journal of …, 2014 - Elsevier
The liver has the unique capacity to regenerate in response to a damaging event. Liver
regeneration is hereby largely driven by hepatocyte proliferation, which in turn relies on cell …

Extracorporeal tissue engineered liver-assist devices

ES Tzanakakis, DJ Hess, TD Sielaff… - Annual review of …, 2000 - annualreviews.org
▪ Abstract The treatment of acute liver failure has evolved to the current concept of hybrid
bioartificial liver (BAL) support, because wholly artificial systems have not proved …

Establishment and characterization of differentiated, nontransformed hepatocyte cell lines derived from mice transgenic for transforming growth factor alpha.

JC Wu, G Merlino, N Fausto - Proceedings of the National …, 1994 - National Acad Sciences
Hepatocytes are extensively used in studies of gene regulation but cannot be maintained in
long-term culture as replicating, differentiated cells while remaining nontumorigenic. We …

[图书][B] Laboratory techniques in biochemistry and molecular biology

TR Flotte, KI Berns - 1969 - books.google.com
Adeno-associated viruses (AAV) are ubiquitous in the human population (> 90% of adults
are seropositive) but have never been implicated as the cause of any disease. They were …

Immunobiology and pathogenesis of hepatocellular injury in hepatitis B virus transgenic mice

T Moriyama, S Guilhot, K Klopchin, B Moss, CA Pinkert… - Science, 1990 - science.org
The role of the immune response to hepatitis B virus (HBV)-encoded antigens in the
pathogenesis of liver cell injury has not been defined because of the absence of appropriate …

[图书][B] Isolated Hepatocytes: Preparation, Properties and Applications: Preparation, Properties and Applications

MN Berry, GJ Barritt, AM Edwards - 1991 - books.google.com
Page 1 LABORATORY TECHNIOUES |N BIOCHEMISTRY AND MOLECULAR BIOLOGY
VOLUME, 21 RH BURDON and PH van KNIPPENBERG Editors isolated hepatocytes …

Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens.

AM Pfeifer, KE Cole, DT Smoot… - Proceedings of the …, 1993 - National Acad Sciences
Normal human liver tissue and cultured human hepatocytes are valuable models to study
xenobiotic metabolism and toxicity, but they only have a limited in vitro life-span and are not …

[HTML][HTML] Human papillomavirus type 16 E6 and E 7 proteins alter NF-kB in cultured cervical epithelial cells and inhibition of NF-kB promotes cell growth and …

ER Vandermark, KA Deluca, CR Gardner, DF Marker… - Virology, 2012 - Elsevier
The NF-kB family of transcription factors regulates important biological functions including
cell growth, survival and the immune response. We found that Human Papillomavirus type …

Altered mitochondrial function and overgeneration of reactive oxygen species precede the induction of apoptosis by 1-O-octadecyl-2-methyl-rac-glycero-3 …

AS Vrablic, CD Albright, CN Craciunescu… - Faseb journal, 2001 - cdr.lib.unc.edu
The mechanism of induction of apoptosis by the novel anti-cancer drug 1-O-octadecyl-2-
methyl-rac-glycero-3-phosphocholine (ET-18-OCH3) was investigated in p53-defective …

Immortalized human hepatocytes as a tool for the study of hepatocytic (de-) differentiation

IJ Schippers, H Moshage, H Roelofsen, M Müller… - Cell biology and …, 1997 - Springer
Primary human hepatocytes were immortalized by stable transfection with a recombinant
plasmid containing the early region of simian virus (SV) 40. The cells were cultured in serum …