Advances in mammalian cell line development technologies for recombinant protein production
T Lai, Y Yang, SK Ng - Pharmaceuticals, 2013 - mdpi.com
From 2006 to 2011, an average of 15 novel recombinant protein therapeutics have been
approved by US Food and Drug Administration (FDA) annually. In addition, the expiration of …
approved by US Food and Drug Administration (FDA) annually. In addition, the expiration of …
An'omics approach towards CHO cell engineering
P Datta, RJ Linhardt… - Biotechnology and …, 2013 - Wiley Online Library
Chinese hamster ovarian cells (CHO) cells have been extensively utilized for industrial
production of biopharmaceutical products, such as monoclonal antibodies, human growth …
production of biopharmaceutical products, such as monoclonal antibodies, human growth …
[HTML][HTML] Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome
Chinese hamster ovary (CHO) cells, first isolated in 1957, are the preferred production host
for many therapeutic proteins. Although genetic heterogeneity among CHO cell lines has …
for many therapeutic proteins. Although genetic heterogeneity among CHO cell lines has …
[HTML][HTML] CHO microRNA engineering is growing up: Recent successes and future challenges
Abstract microRNAs with their ability to regulate complex pathways that control cellular
behavior and phenotype have been proposed as potential targets for cell engineering in the …
behavior and phenotype have been proposed as potential targets for cell engineering in the …
[HTML][HTML] Stable overexpression of miR-17 enhances recombinant protein production of CHO cells
Abstract miRNAs negatively regulate gene expression at post-transcriptional level, and
consequently play an important role in the control of many cellular pathways. The use of …
consequently play an important role in the control of many cellular pathways. The use of …
[HTML][HTML] Stable microRNA expression enhances therapeutic antibody productivity of Chinese hamster ovary cells
M Strotbek, L Florin, J Koenitzer, A Tolstrup… - Metabolic …, 2013 - Elsevier
MicroRNAs (miRNAs) are short non-coding RNAs that post-transcriptionally regulate the
expression of different target genes and, thus, enable engineered gene networks to achieve …
expression of different target genes and, thus, enable engineered gene networks to achieve …
miRNA engineering of CHO cells facilitates production of difficult‐to‐express proteins and increases success in cell line development
S Fischer, KF Marquart, LA Pieper… - Biotechnology and …, 2017 - Wiley Online Library
In recent years, coherent with growing biologics portfolios also the number of complex and
thus difficult‐to‐express (DTE) therapeutic proteins has increased considerably. DTE …
thus difficult‐to‐express (DTE) therapeutic proteins has increased considerably. DTE …
A functional high‐content miRNA screen identifies miR‐30 family to boost recombinant protein production in CHO cells
S Fischer, T Buck, A Wagner, C Ehrhart… - Biotechnology …, 2014 - Wiley Online Library
The steady improvement of mammalian cell factories for the production of
biopharmaceuticals is a key challenge for the biotechnology community. Recently, small …
biopharmaceuticals is a key challenge for the biotechnology community. Recently, small …
Modulation of mammalian translation by a ribosome-associated tRNA half
Originally considered futile degradation products, tRNA-derived RNA fragments (tdRs) have
been shown over the recent past to be crucial players in orchestrating various cellular …
been shown over the recent past to be crucial players in orchestrating various cellular …
Tracing production instability in a clonally derived CHO cell line using single‐cell transcriptomics
I Tzani, N Herrmann, S Carillo… - Biotechnology and …, 2021 - Wiley Online Library
A variety of mechanisms including transcriptional silencing, gene copy loss, and increased
susceptibility to cellular stress have been associated with a sudden or gradual loss of …
susceptibility to cellular stress have been associated with a sudden or gradual loss of …