Advances in mammalian cell line development technologies for recombinant protein production

T Lai, Y Yang, SK Ng - Pharmaceuticals, 2013 - mdpi.com
From 2006 to 2011, an average of 15 novel recombinant protein therapeutics have been
approved by US Food and Drug Administration (FDA) annually. In addition, the expiration of …

An'omics approach towards CHO cell engineering

P Datta, RJ Linhardt… - Biotechnology and …, 2013 - Wiley Online Library
Chinese hamster ovarian cells (CHO) cells have been extensively utilized for industrial
production of biopharmaceutical products, such as monoclonal antibodies, human growth …

[HTML][HTML] Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome

NE Lewis, X Liu, Y Li, H Nagarajan, G Yerganian… - Nature …, 2013 - nature.com
Chinese hamster ovary (CHO) cells, first isolated in 1957, are the preferred production host
for many therapeutic proteins. Although genetic heterogeneity among CHO cell lines has …

[HTML][HTML] CHO microRNA engineering is growing up: Recent successes and future challenges

V Jadhav, M Hackl, A Druz, S Shridhar, CY Chung… - Biotechnology …, 2013 - Elsevier
Abstract microRNAs with their ability to regulate complex pathways that control cellular
behavior and phenotype have been proposed as potential targets for cell engineering in the …

[HTML][HTML] Stable overexpression of miR-17 enhances recombinant protein production of CHO cells

V Jadhav, M Hackl, G Klanert, JAH Bort, R Kunert… - Journal of …, 2014 - Elsevier
Abstract miRNAs negatively regulate gene expression at post-transcriptional level, and
consequently play an important role in the control of many cellular pathways. The use of …

[HTML][HTML] Stable microRNA expression enhances therapeutic antibody productivity of Chinese hamster ovary cells

M Strotbek, L Florin, J Koenitzer, A Tolstrup… - Metabolic …, 2013 - Elsevier
MicroRNAs (miRNAs) are short non-coding RNAs that post-transcriptionally regulate the
expression of different target genes and, thus, enable engineered gene networks to achieve …

miRNA engineering of CHO cells facilitates production of difficult‐to‐express proteins and increases success in cell line development

S Fischer, KF Marquart, LA Pieper… - Biotechnology and …, 2017 - Wiley Online Library
In recent years, coherent with growing biologics portfolios also the number of complex and
thus difficult‐to‐express (DTE) therapeutic proteins has increased considerably. DTE …

A functional high‐content miRNA screen identifies miR‐30 family to boost recombinant protein production in CHO cells

S Fischer, T Buck, A Wagner, C Ehrhart… - Biotechnology …, 2014 - Wiley Online Library
The steady improvement of mammalian cell factories for the production of
biopharmaceuticals is a key challenge for the biotechnology community. Recently, small …

Modulation of mammalian translation by a ribosome-associated tRNA half

Y Gonskikh, M Gerstl, M Kos, N Borth, M Schosserer… - RNA biology, 2020 - Taylor & Francis
Originally considered futile degradation products, tRNA-derived RNA fragments (tdRs) have
been shown over the recent past to be crucial players in orchestrating various cellular …

Tracing production instability in a clonally derived CHO cell line using single‐cell transcriptomics

I Tzani, N Herrmann, S Carillo… - Biotechnology and …, 2021 - Wiley Online Library
A variety of mechanisms including transcriptional silencing, gene copy loss, and increased
susceptibility to cellular stress have been associated with a sudden or gradual loss of …