Hydroxyl radical protein footprinting (HRPF) coupled to mass spectrometry has been
successfully used to investigate a plethora of protein-related questions. The method, which …

“Footprinting” refers to assays that examine ligand binding and conformational changes by
determining the solvent accessibility of the backbone, bases, or side chain structures of …

Targeted cancer therapies, which act on specific cancer-associated molecular targets, are
predominantly inhibitors of oncogenic kinases. While these drugs have achieved some …

Mass spectrometry (MS) is increasingly being used to probe the structure and dynamics of
proteins and the complexes they form with other macromolecules. There are now several …

Footprinting of proteins by hydroxyl radicals generated on the millisecond to minute
timescales to probe protein surfaces suffers from the uncertainty that radical reactions cause …

Antibodies, also known as immunoglobulins, have emerged as one of the most promising
classes of therapeutics in the biopharmaceutical industry. The need for complete …

Structural proteomics approaches using mass spectrometry are increasingly used in biology
to examine the composition and structure of macromolecules. Hydroxyl radical–mediated …

Fast photochemical oxidation of proteins (FPOP) is a chemical footprinting method whereby
exposed amino-acid residues are covalently labeled by oxidation with hydroxyl radicals …

Using mass spectrometry (MS) to obtain information about a higher order structure of protein
requires that a protein's structural properties are encoded into the mass of that protein …

Protein surfaces are important in most biological processes, including protein− protein
interactions, enzymatic catalysis, and protein− ligand binding. We report a method in which …