Host cell proteases: critical determinants of coronavirus tropism and pathogenesis

JK Millet, GR Whittaker - Virus research, 2015 - Elsevier
Coronaviruses are a large group of enveloped, single-stranded positive-sense RNA viruses
that infect a wide range of avian and mammalian species, including humans. The …

[HTML][HTML] Physiological and molecular triggers for SARS-CoV membrane fusion and entry into host cells

JK Millet, GR Whittaker - Virology, 2018 - Elsevier
During viral entry, enveloped viruses require the fusion of their lipid envelope with host cell
membranes. For coronaviruses, this critical step is governed by the virally-encoded spike (S) …

[HTML][HTML] Coronavirus entry: how we arrived at SARS-CoV-2

GR Whittaker, S Daniel, JK Millet - Current opinion in virology, 2021 - Elsevier
Because of the COVID-19 pandemic, the novel coronavirus SARS-CoV-2 has risen to shape
scientific research during 2020, with its spike (S) protein being a predominant focus. The S …

HIV virions sense plasma membrane heterogeneity for cell entry

ST Yang, AJB Kreutzberger, V Kiessling… - Science …, 2017 - science.org
It has been proposed that cholesterol in host cell membranes plays a pivotal role for cell
entry of HIV. However, it remains largely unknown why virions prefer cholesterol-rich …

Supramolecular architecture of the coronavirus particle

BW Neuman, MJ Buchmeier - Advances in virus research, 2016 - Elsevier
Coronavirus particles serve three fundamentally important functions in infection. The virion
provides the means to deliver the viral genome across the plasma membrane of a host cell …

Imaging, tracking and computational analyses of virus entry and egress with the cytoskeleton

IH Wang, CJ Burckhardt, A Yakimovich, UF Greber - Viruses, 2018 - mdpi.com
Viruses have a dual nature: particles are “passive substances” lacking chemical energy
transformation, whereas infected cells are “active substances” turning-over energy. How …

Production of pseudotyped particles to study highly pathogenic coronaviruses in a biosafety level 2 setting

JK Millet, T Tang, L Nathan, JA Jaimes… - Journal of …, 2019 - pmc.ncbi.nlm.nih.gov
The protocol aims to generate coronavirus (CoV) spike (S) fusion protein pseudotyped
particles with a murine leukemia virus (MLV) core and luciferase reporter, using a simple …

Functional infectious nanoparticle detector: Finding viruses by detecting their host entry functions using organic bioelectronic devices

T Tang, A Savva, WC Traberg, C Xu, Q Thiburce… - ACS …, 2021 - ACS Publications
Emerging viruses will continue to be a threat to human health and wellbeing into the
foreseeable future. The COVID-19 pandemic revealed the necessity for rapid viral sensing …

[HTML][HTML] Engineering a model to study viral infections: bioprinting, microfluidics, and organoids to defeat coronavirus disease 2019 (COVID-19)

A Shpichka, P Bikmulina, M Peshkova… - … journal of bioprinting, 2020 - ncbi.nlm.nih.gov
While the number of studies related to severe acute respiratory syndrome-related
coronavirus 2 (SARS-CoV-2) is constantly growing, it is essential to provide a framework of …

Membrane protein mobility and orientation preserved in supported bilayers created directly from cell plasma membrane blebs

MJ Richards, CY Hsia, RR Singh, H Haider, J Kumpf… - Langmuir, 2016 - ACS Publications
Membrane protein interactions with lipids are crucial for their native biological behavior, yet
traditional characterization methods are often carried out on purified protein in the absence …