Lentiviral vectors are very efficient at transducing dividing and quiescent cells, which makes
them highly useful tools for genetic analysis and gene therapy. Traditionally this efficiency …

Publisher Summary This chapter focuses on the two aspects of the in vivo structure and
function of retroviral integrase (IN). Experiments that defined the viral att sites are discussed …

The integration of a DNA copy of the human immunodeficiency virus type 1 (HIV-1) genome
into a chromosome of an infected cell is a pivotal step in virus replication. Integration …

HIV-1 replication depends on the viral enzyme integrase that mediates integration of a DNA
copy of the virus into the host cell genome. This enzyme represents a novel target to which …

The viral integrase (IN) protein is the only viral protein known to be required for integration of
the human immunodeficiency virus type 1 (HIV-1) genome into the host cell DNA, a step in …

Integrase is the only viral protein necessary for integration of retroviral DNA into
chromosomal DNA of the host cell. Biochemical analysis of human immunodeficiency virus …

Abstract Analysis of the crystal structure of HIV‐1 integrase reveals a cluster of lysine
residues near the active site. Using site‐directed mutagenesis and photo‐crosslinking we …

The yeast retroviruslike element Ty3 inserts at the transcription initiation sites of genes
transcribed by RNA polymerase III (Pol III). An in vitro integration assay was developed with …

Retroviral integrase catalyzes the essential step of integrating a double-stranded DNA copy
of the viral genome into a host cell chromosome. Mutational studies have revealed that …

It has been recently demonstrated that the Mg2+-dependent 3 '-processing activity of purified
human immunodeficiency virus type-1 (HIV-1) integrase is stimulated by the addition of …