Human topoisomerases and their roles in genome stability and organization

Y Pommier, A Nussenzweig, S Takeda… - … Reviews Molecular Cell …, 2022 - nature.com
Human topoisomerases comprise a family of six enzymes: two type IB (TOP1 and
mitochondrial TOP1 (TOP1MT), two type IIA (TOP2A and TOP2B) and two type IA (TOP3A …

Untangling the roles of TOP2A and TOP2B in transcription and cancer

L Uusküla-Reimand, MD Wilson - Science advances, 2022 - science.org
Type II topoisomerases (TOP2) are conserved regulators of chromatin topology that catalyze
reversible DNA double-strand breaks (DSBs) and are essential for maintaining genomic …

Roles of eukaryotic topoisomerases in transcription, replication and genomic stability

Y Pommier, Y Sun, SN Huang, JL Nitiss - Nature reviews Molecular cell …, 2016 - nature.com
Topoisomerases introduce transient DNA breaks to relax supercoiled DNA, remove
catenanes and enable chromosome segregation. Human cells encode six topoisomerases …

Genome organization drives chromosome fragility

A Canela, Y Maman, S Jung, N Wong, E Callen, A Day… - Cell, 2017 - cell.com
In this study, we show that evolutionarily conserved chromosome loop anchors bound by
CCCTC-binding factor (CTCF) and cohesin are vulnerable to DNA double strand breaks …

Anthracyclines as topoisomerase II poisons: from early studies to new perspectives

J Marinello, M Delcuratolo, G Capranico - International journal of …, 2018 - mdpi.com
Mammalian DNA topoisomerases II are targets of anticancer anthracyclines that act by
stabilizing enzyme-DNA complexes wherein DNA strands are cut and covalently linked to …

Drugging topoisomerases: lessons and challenges

Y Pommier - ACS chemical biology, 2013 - ACS Publications
Topoisomerases are ubiquitous enzymes that control DNA supercoiling and entanglements.
They are essential during transcription and replication, and topoisomerase inhibitors are …

Topoisomerase II-induced chromosome breakage and translocation is determined by chromosome architecture and transcriptional activity

A Canela, Y Maman, NH Shar-yin, G Wutz, W Tang… - Molecular cell, 2019 - cell.com
Topoisomerase II (TOP2) relieves torsional stress by forming transient cleavage complex
intermediates (TOP2ccs) that contain TOP2-linked DNA breaks (DSBs). While TOP2ccs are …

New mechanistic and functional insights into DNA topoisomerases

SH Chen, NL Chan, T Hsieh - Annual review of biochemistry, 2013 - annualreviews.org
DNA topoisomerases are nature's tools for resolving the unique problems of DNA
entanglement that occur owing to unwinding and rewinding of the DNA helix during …

Topoisomerase II beta interacts with cohesin and CTCF at topological domain borders

L Uusküla-Reimand, H Hou, P Samavarchi-Tehrani… - Genome biology, 2016 - Springer
Abstract Background Type II DNA topoisomerases (TOP2) regulate DNA topology by
generating transient double stranded breaks during replication and transcription …

Spatial chromosome folding and active transcription drive DNA fragility and formation of oncogenic MLL translocations

HJ Gothe, BAM Bouwman, EG Gusmao, R Piccinno… - Molecular cell, 2019 - cell.com
How spatial chromosome organization influences genome integrity is still poorly understood.
Here, we show that DNA double-strand breaks (DSBs) mediated by topoisomerase 2 (TOP2) …