The defining trait of obligate anaerobes is that oxygen blocks their growth, yet the underlying
mechanisms are unclear. A popular hypothesis was that these microorganisms failed to …

Bacterial microcompartments (MCPs) are proteinaceous organelles consisting of a
metabolic pathway encapsulated within a selectively permeable protein shell. Hundreds of …

Enzymes of the radical S-adenosyl-l-methionine (radical SAM, RS) superfamily, the largest
in nature, catalyze remarkably diverse reactions initiated by H-atom abstraction. Glycyl …

Modulation of cell death is a powerful strategy employed by pathogenic bacteria to evade
host immune clearance and occupy profitable replication niches during infection …

Oxygen-sensitive metalloenzymes are responsible for many of the most fundamental
biochemical processes in nature, from the reduction of dinitrogen in nitrogenase to the …

Pyruvate formate-lyase (PFL) is a glycyl radical enzyme (GRE) that converts pyruvate and
coenzyme A into acetyl-CoA and formate in a reaction that is crucial to the primary …

Results Trapping the Organometallic Intermediate Ω During Reaction of PFL-AE, SAM and G-
pep. RFQ (Rapid freeze-quench) EPR measurements were carried out over a range of short …

The reaction of benzylsuccinate synthase, the radical-based addition of toluene to a
fumarate cosubstrate, is initiated by hydrogen transfer from a conserved cysteine to the …

During glucose fermentation, Escherichia coli and many other microorganisms employ the
glycyl radical enzyme (GRE) pyruvate formate-lyase (PflB) to catalyze the coenzyme A …

Pyruvate formate-lyase (PFL) is a glycyl radical enzyme (GRE) playing a pivotal role in the
metabolism of strict and facultative anaerobes. Its activation is carried out by a PFL …