State-of-the-art tissue-clearing methods provide subcellular-level optical access to intact
tissues from individual organs and even to some entire mammals. When combined with light …

Tissue clearing of gross anatomical samples was first described more than a century ago
and has only recently found widespread use in the field of microscopy. This renaissance has …

The segmented body plan of vertebrates is established during somitogenesis, a well-studied
process in model organisms; however, the details of this process in humans remain largely …

Adeno-associated viruses (AAVs) are commonly used for in vivo gene transfer.
Nevertheless, AAVs that provide efficient transduction across specific organs or cell …

Abstract Analysis of entire transparent rodent bodies after clearing could provide holistic
biological information in health and disease, but reliable imaging and quantification of …

Many biological investigations require 3D imaging of cells or tissues with nanoscale spatial
resolution. We recently discovered that preserved biological specimens can be physically …

Animal models of human diseases that accurately recapitulate clinical pathology are
indispensable for understanding molecular mechanisms and advancing preclinical studies …

Modern optogenetics can be tuned to evoke activity that corresponds to naturally occurring
local or global activity in timing, magnitude or individual-cell patterning. This outcome has …

Astrocytes are ubiquitous in the brain and are widely held to be largely identical. However,
this view has not been fully tested, and the possibility that astrocytes are neural circuit …

The skin is a multilayered organ, equipped with appendages (that is, follicles and glands),
that is critical for regulating body temperature and the retention of bodily fluids, guarding …