Flow cytometry: basic principles and applications

A Adan, G Alizada, Y Kiraz, Y Baran… - Critical reviews in …, 2017 - Taylor & Francis
Flow cytometry is a sophisticated instrument measuring multiple physical characteristics of a
single cell such as size and granularity simultaneously as the cell flows in suspension …

Understanding FRET as a research tool for cellular studies

D Shrestha, A Jenei, P Nagy, G Vereb… - International journal of …, 2015 - mdpi.com
Communication of molecular species through dynamic association and/or dissociation at
various cellular sites governs biological functions. Understanding these physiological …

[HTML][HTML] Overcoming limitations of FRET measurements

SJ Leavesley, TC Rich - Cytometry. Part A: the journal of the …, 2016 - ncbi.nlm.nih.gov
Förster resonance energy transfer (FRET) is a non-radiative process that has been
extensively applied for probing molecular events involved in a range of cellular and …

A guide to flow cytometry: components, basic principles, experimental design, and cancer research applications

L El‐Hajjar, F Ali Ahmad, R Nasr - Current Protocols, 2023 - Wiley Online Library
Flow cytometry (FCM) is a state‐of‐the‐art technique for the qualitative and quantitative
assessment of cells and other particles' physical and biological properties. These cells are …

Milestones in the development and implementation of FRET-based sensors of intracellular signals: a biological perspective of the history of FRET

J Deal, DJ Pleshinger, SC Johnson, SJ Leavesley… - Cellular signalling, 2020 - Elsevier
Fӧrster resonance energy transfer (FRET) has been described for more than a century.
FRET has become a mainstay for the study of protein localization in living cells and tissues …

Improving quality, reproducibility, and usability of FRET‐based tension sensors

EM Gates, AS LaCroix, KE Rothenberg… - Cytometry Part …, 2019 - Wiley Online Library
Mechanobiology, the study of how mechanical forces affect cellular behavior, is an emerging
field of study that has garnered broad and significant interest. Researchers are currently …

Principles of resonance energy transfer

Á Szabó, J Szöllősi, P Nagy - Current protocols, 2022 - Wiley Online Library
This unit describes the basic principles of Förster resonance energy transfer (FRET).
Beginning with a brief summary of the history of FRET applications, the theory of FRET is …

Hyperspectral imaging fluorescence excitation scanning for colon cancer detection

SJ Leavesley, M Walters, C Lopez… - … of biomedical optics, 2016 - spiedigitallibrary.org
Optical spectroscopy and hyperspectral imaging have shown the potential to discriminate
between cancerous and noncancerous tissue with high sensitivity and specificity. However …

Quantifying macromolecular interactions in living cells using FRET two-hybrid assays

ES Butz, M Ben-Johny, M Shen, PS Yang, L Sang… - Nature protocols, 2016 - nature.com
Förster resonance energy transfer (FRET) is a versatile method for analyzing protein–protein
interactions within living cells. This protocol describes a nondestructive live-cell FRET assay …

Excitation-scanning hyperspectral imaging microscope

PF Favreau, C Hernandez, T Heaster… - … of biomedical optics, 2014 - spiedigitallibrary.org
Hyperspectral imaging is a versatile tool that has recently been applied to a variety of
biomedical applications, notably live-cell and whole-tissue signaling. Traditional …