Crop biofortification for iron (Fe), zinc (Zn) and vitamin A with transgenic approaches

S Kumar, A Palve, C Joshi, RK Srivastava - Heliyon, 2019 - cell.com
Micronutrient malnutrition is an important issue in the developing countries especially in
Asia and Africa where millions of school-going children and pregnant women are affected …

Engineering complex metabolic pathways in plants

G Farré, D Blancquaert, T Capell… - Annual Review of …, 2014 - annualreviews.org
Metabolic engineering can be used to modulate endogenous metabolic pathways in plants
or introduce new metabolic capabilities in order to increase the production of a desirable …

Robust transcriptional activation in plants using multiplexed CRISPR-Act2. 0 and mTALE-Act systems

LG Lowder, J Zhou, Y Zhang, A Malzahn, Z Zhong… - Molecular Plant, 2018 - cell.com
User-friendly tools for robust transcriptional activation of endogenous genes are highly
demanded in plants. We previously showed that a dCas9-VP64 system consisting of the …

Particle bombardment technology and its applications in plants

II Ozyigit, K Yucebilgili Kurtoglu - Molecular biology reports, 2020 - Springer
Particle bombardment, or biolistics, has emerged as an excellent alternative approach for
plant genetic transformation which circumvents the limitations of Agrobacterium-mediated …

Recent progress and future prospect of CRISPR/Cas-derived transcription activation (CRISPRa) system in plants

X Ding, L Yu, L Chen, Y Li, J Zhang, H Sheng, Z Ren… - Cells, 2022 - mdpi.com
Genome editing technology has become one of the hottest research areas in recent years.
Among diverse genome editing tools, the Clustered Regularly Interspaced Short …

Biodegradation of p-nitrophenol by engineered strain

J Xu, B Wang, W Zhang, FJ Zhang, Y Deng, Y Wang… - AMB Express, 2021 - Springer
Abstract p-Nitrophenol (PNP) is an important environmental pollutant and can causes
significant environmental and health risks. Compared with the traditional methods …

Gene pyramiding in transgenic plant development: approaches and challenges

R Rajput, J Naik, P Misra, PK Trivedi… - Journal of Plant Growth …, 2023 - Springer
Climate change leads to frequent alterations in environmental factors with a reciprocal
impact on crop productivity. Over the last few decades, various approaches have been used …

Synthetic biology for production of natural and new‐to‐nature terpenoids in photosynthetic organisms

P Arendt, J Pollier, N Callewaert… - The Plant …, 2016 - Wiley Online Library
With tens of thousands of characterized members, terpenoids constitute the largest class of
natural compounds that are synthesized by all living organisms. Several terpenoids play …

Multiplex and optimization of dCas9‐TV‐mediated gene activation in plants

X Xiong, J Liang, Z Li, BQ Gong… - Journal of Integrative Plant …, 2021 - Wiley Online Library
Synthetic gene activators consisting of nuclease‐dead Cas9 (dCas9) for single‐guide RNA
(sgRNA)‐directed promoter binding and a transcriptional activation domain (TAD) represent …

Recent developments in therapeutic protein expression technologies in plants

S Fahad, FA Khan, NS Pandupuspitasari… - Biotechnology …, 2015 - Springer
Infectious diseases and cancers are some of the commonest causes of deaths throughout
the world. The previous two decades have witnessed a combined endeavor across various …