Deciphering the multi-scale, quantitative cis-regulatory code

S Kim, J Wysocka - Molecular cell, 2023 - cell.com
Uncovering the cis-regulatory code that governs when and how much each gene is
transcribed in a given genome and cellular state remains a central goal of biology. Here, we …

Transcription factor–DNA binding: beyond binding site motifs

S Inukai, KH Kock, ML Bulyk - Current opinion in genetics & development, 2017 - Elsevier
Sequence-specific transcription factors (TFs) regulate gene expression by binding to cis-
regulatory elements in promoter and enhancer DNA. While studies of TF–DNA binding have …

Evolutionary constraint and innovation across hundreds of placental mammals

MJ Christmas, IM Kaplow, DP Genereux, MX Dong… - Science, 2023 - science.org
Zoonomia is the largest comparative genomics resource for mammals produced to date. By
aligning genomes for 240 species, we identify bases that, when mutated, are likely to affect …

Potent and uniform fetal hemoglobin induction via base editing

T Mayuranathan, GA Newby, R Feng, Y Yao… - Nature …, 2023 - nature.com
Inducing fetal hemoglobin (HbF) in red blood cells can alleviate β-thalassemia and sickle
cell disease. We compared five strategies in CD34+ hematopoietic stem and progenitor …

Expanded encyclopaedias of DNA elements in the human and mouse genomes

JE Moore, MJ Purcaro, HE Pratt, CB Epstein… - Nature, 2020 - nature.com
The human and mouse genomes contain instructions that specify RNAs and proteins and
govern the timing, magnitude, and cellular context of their production. To better delineate …

A stromal Integrated Stress Response activates perivascular cancer-associated fibroblasts to drive angiogenesis and tumour progression

II Verginadis, H Avgousti, J Monslow, G Skoufos… - Nature cell …, 2022 - nature.com
Bidirectional signalling between the tumour and stroma shapes tumour aggressiveness and
metastasis. ATF4 is a major effector of the Integrated Stress Response, a homeostatic …

Base-resolution models of transcription-factor binding reveal soft motif syntax

Ž Avsec, M Weilert, A Shrikumar, S Krueger… - Nature …, 2021 - nature.com
The arrangement (syntax) of transcription factor (TF) binding motifs is an important part of the
cis-regulatory code, yet remains elusive. We introduce a deep learning model, BPNet, that …

Global reference mapping of human transcription factor footprints

J Vierstra, J Lazar, R Sandstrom, J Halow, K Lee… - Nature, 2020 - nature.com
Combinatorial binding of transcription factors to regulatory DNA underpins gene regulation
in all organisms. Genetic variation in regulatory regions has been connected with diseases …

hTFtarget: a comprehensive database for regulations of human transcription factors and their targets

Q Zhang, W Liu, HM Zhang, GY Xie… - Genomics …, 2020 - academic.oup.com
Transcription factors (TFs) as key regulators play crucial roles in biological processes. The
identification of TF–target regulatory relationships is a key step for revealing functions of TFs …

SARS-CoV-2 receptor ACE2 is an interferon-stimulated gene in human airway epithelial cells and is detected in specific cell subsets across tissues

CGK Ziegler, SJ Allon, SK Nyquist, IM Mbano, VN Miao… - Cell, 2020 - cell.com
There is pressing urgency to understand the pathogenesis of the severe acute respiratory
syndrome coronavirus clade 2 (SARS-CoV-2), which causes the disease COVID-19. SARS …