The challenges of sample preparation can limit a researcher's selection of transmission
electron microcopy (TEM) for analysis of yeast. However, with the exception of thin …

Contact sites are areas of close apposition between two membranes that coordinate
nonvesicular communication between organelles. Such interactions serve a wide range of …

NEM prevents mitotic reassembly of Golgi cisternae into stacked structures. The major target
of NEM is a 65 kDa protein conserved from yeast to mammals. Antibodies to this protein and …

There is a debate over how protein trafficking is performed through the Golgi apparatus,,,. In
the secretory pathway, secretory proteins that are synthesized in the endoplasmic reticulum …

Golgi stacks are often located near sites of “transitional ER”(tER), where COPII transport
vesicles are produced. This juxtaposition may indicate that Golgi cisternae form at tER sites …

In vitro studies have suggested that Golgi stack formation involves two homologous
peripheral Golgi proteins, GRASP65 and GRASP55, which localize to the cis and medial …

Small GTPases of the Ypt/rab family are involved in the regulation of vesicular transport.
These GTPases apparently function during the targeting of vesicles to the acceptor …

In eukaryotic cells, monomeric GTPases of the Ypt/Rab family function as regulators at
defined steps of vesicular transport in exo‐and endocytosis. Here we report on the isolation …

The Cvt pathway is a biosynthetic transport route for a distinct subset of resident yeast
vacuolar hydrolases, whereas macroautophagy is a nonspecific degradative mechanism …

The budding yeast Pichia pastoris contains ordered Golgi stacks next to discrete transitional
endoplasmic reticulum (tER) sites, making this organism ideal for structure–function studies …